In this study, nano-carbonated hydroxyapatite (n-CHAp) was successfully synthesized with abalone shells (Halioitis asinina) as the calcium source using precipitation methods with aging time variations, namely, 0 (without the aging process), 24, and 48 h. Based on an analysis of X-ray diffraction characterization, the spectrum of the n-CHAp is shown for all sample variations in aging time. The results of the calculation of lattice parameter values confirm that the phase formed is the B-type CHAp phase with the increasing crystallinity degree, crystallite size, particle size, and polydispersity which is confirmed by the presence of the CO₃^2- functional group at 1438 cm⁻¹ and 878 cm⁻¹, that is, the B-type carbonate substitution characteristic. The presence of the carbonate ions identified as smaller during the extension of aging time causes the decreasing value of the Ca/P mole ratio but still has a value greater than the HAp Ca/P value (1.67), which is 1.80–1.72. Based on the transmission electron microscopy analysis, the nanometer-size of B-type CHAp particles was successfully obtained. According to the criteria for nanostructures, crystallographic properties, carbonate content, and chemical processes, B-type CHAp samples based on abalone shells (Halioitis asinina) are one of the candidates in bioceramics for bone tissue engineering applications.

本研究以鲍鱼壳（Halioitis asinina）为钙源，采用具有老化时间变化的沉淀法成功合成纳米碳酸羟基磷灰石（n-CHAp），即0（无老化过程）、24小时和48小时。 . 基于对 X 射线衍射特征的分析，显示了所有样品老化时间变化的 n-CHAp 光谱。晶格参数值的计算结果证实，形成的相是 B 型 CHAp 相，随着结晶度、微晶尺寸、粒径和多分散性的增加，CO ₃ ^2-官能团的存在证实了这一点。 1438 厘米^-1和 878 厘米^-1，即B型碳酸酯取代特性。在老化时间延长期间被确定为较小的碳酸根离子的存在导致 Ca/P 摩尔比值的降低，但仍具有大于 HAp Ca/P 值 (1.67) 的值，即 1.80–1.72。基于透射电镜分析，成功获得了纳米级的B型CHAP颗粒。根据纳米结构、晶体学特性、碳酸盐含量和化学过程的标准，基于鲍鱼壳 ( Halioitis asinina ) 的B 型 CHAp 样品是骨组织工程应用生物陶瓷的候选材料之一。