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Shugoshin ensures maintenance of the spindle assembly checkpoint response and efficient spindle disassembly
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-08-18 , DOI: 10.1111/mmi.14796
Aakanksha Sane 1 , Shreyas Sridhar 2, 3 , Kaustuv Sanyal 2, 3 , Santanu K Ghosh 1
Affiliation  

Shugoshin proteins are evolutionarily conserved across eukaryotes, with some species-specific cellular functions, ensuring the fidelity of chromosome segregation. They act as adaptors at various subcellular locales to mediate several protein-protein interactions in a spatio-temporal manner. Here, we characterize shugoshin (Sgo1) in the human fungal pathogen Candida albicans. We observe that Sgo1 retains its centromeric localization and performs its conserved functions of regulating the sister chromatid biorientation, centromeric condensin localization, and maintenance of chromosomal passenger complex (CPC). We identify novel roles of Sgo1 as a spindle assembly checkpoint (SAC) component with functions in maintaining a prolonged SAC response by retaining Mad2 and Bub1 at the kinetochores in response to improper kinetochore-microtubule attachments. Strikingly, we discover the in vivo localization of Sgo1 along the length of the mitotic spindle. Our results indicate that Sgo1 performs a hitherto unknown function of facilitating timely disassembly of the mitotic spindle in C. albicans. To summarize, this study unravels a unique functional adaptation of shugoshin in maintaining genomic stability.

中文翻译:

Shugoshin 确保维护主轴组件检查点响应和高效的主轴拆卸

Shugoshin 蛋白在真核生物中是进化上保守的,具有一些物种特异性的细胞功能,确保了染色体分离的保真度。它们充当各种亚细胞区域的适配器,以时空方式介导几种蛋白质-蛋白质相互作用。在这里,我们描述了人类真菌病原体白色念珠菌中的 shugoshin (Sgo1). 我们观察到 Sgo1 保留其着丝粒定位并执行其调节姐妹染色单体生物定向、着丝粒凝聚素定位和维持染色体乘客复合体 (CPC) 的保守功能。我们确定 Sgo1 作为纺锤体装配检查点 (SAC) 组件的新作用,其功能是通过在动粒处保留 Mad2 和 Bub1 以响应不正确的动粒 - 微管附件来维持延长的 SAC 响应。引人注目的是,我们发现 Sgo1 沿着有丝分裂纺锤体的长度在体内定位。我们的研究结果表明,Sgo1 具有前所未有的促进白色念珠菌有丝分裂纺锤体及时拆卸的功能。. 总而言之,这项研究揭示了 shugoshin 在维持基因组稳定性方面的独特功能适应性。
更新日期:2021-10-25
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