The chlorophyte microalga Chlorella sorokiniana was tested for the bioremediation of heavy metals pollution. It was cultured with different concentrations of Cu⁺²⁺, Cd⁺²⁺, As (III) and As (V), showing a significant inhibition on its growth at concentrations of 500 µM Cu⁺²⁺, 250 µM Cd⁺²⁺, 750 µM AsO₃^3- and 5 mM AsO₄^3- or higher. Moreover, the consumption of ammonium was also studied, showing significant differences for concentrations higher than 1 mM of Cu⁺²⁺ and As (III), and 5 mM of As (V). The determination of intracellular heavy metals concentration revealed that Chlorella sorokiniana is an outstanding Cd accumulator organism, able to accumulate 11,232 mg kg⁻¹ of Cd, and removing 65% of initial concentration of this heavy metal. Finally, antioxidant enzymes, such as catalase (CAT) and ascorbate peroxidase (APX), and enzymes involved in the production of glutamate and cysteine, such as glutamine syntethase (GS), glutamate dehydrogenase (GDH), O-acetylserine (thiol) lyase (OASTL) and NAD-isocitrate dehydrogenase (NAD-IDH) were studied both at gene expression and enzymatic activity levels. These enzymes exhibited different grades of upregulation, especially in response to Cd and As stress. However, GS expression was downregulated when Chlorella sorokiniana was cultured in the presence of these heavy metals.

测试了叶绿微藻Chlorella sorokiniana对重金属污染的生物修复。将其与不同浓度的Cu ^{+2 +}、Cd ^{+2 +}、As (III) 和As (V) 一起培养，在500 µM Cu ^{+2 +}、250 µM Cd ^{+2 +}浓度下对其生长有显着抑制, 750 µM AsO ₃ ^{3 -}和 5 mM AsO ₄ ^{3 -}或更高。此外，还研究了铵的消耗，显示出高于 1 mM Cu ^{+2 +}浓度的显着差异和 As (III)，以及 5 mM 的 As (V)。胞内重金属浓度的测定表明，Chlorella sorokiniana是一种优秀的Cd 蓄积生物，能够蓄积11,232 mg kg ^-1的Cd，去除该重金属初始浓度的65%。最后，抗氧化酶，如过氧化氢酶 (CAT) 和抗坏血酸过氧化物酶 (APX)，以及参与谷氨酸和半胱氨酸生产的酶，如谷氨酰胺合成酶 (GS)、谷氨酸脱氢酶 (GDH)、O-乙酰丝氨酸（硫醇）裂解酶(OASTL) 和 NAD-异柠檬酸脱氢酶 (NAD-IDH) 在基因表达和酶活性水平上进行了研究。这些酶表现出不同程度的上调，尤其是在响应 Cd 和 As 胁迫时。然而，当 GS 表达下调时Chlorella sorokiniana在这些重金属存在下进行培养。