ABSTRACT

The reduced yields of acetaldehyde and fusel alcohols through fermentation by Saccharomyces cerevisiae is of significance for the improvement of the flavor and health of alcoholic beverages. In this study, the ADH2 (encode alcohol dehydrogenase) and THI3 (encode decarboxylase) genes of the industrial diploid strain S. cerevisiae XF1 were deleted. Results showed that single-gene-deletion mutants by separate gene deletion of ADH2 or THI3 led to a reduced production of the acetaldehyde or fusel alcohols, respectively. In the meantime, the double-gene-deletion mutant S. cerevisiae XF1-AT was constructed by deleting the ADH2 and THI3 simultaneously. An equivalent level of the ethanol production by the S. cerevisiae XF1-AT could be achieved but with the yields of acetaldehyde, isoamyl alcohol and iso-butanol reduced by 42.09%, 15.65% and 20.16%, respectively. In addition, there was no interaction between the ADH2 deletion and THI3 deletion in reducing the production of acetaldehyde and fusel alcohols. The engineered S. cerevisiae XF1-AT provided a new strategy to alcoholic beverages brewing industry for reducing the production of acetaldehyde as well as the fusel alcohols.

中文翻译：

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同时删除酿酒酵母的ADH2和THI3基因以降低乙醛和杂醇的产量

摘要

通过酿酒酵母发酵降低乙醛和杂醇的产量对于改善酒精饮料的风味和健康具有重要意义。在本研究中，工业二倍体菌株酿酒酵母XF1的ADH2（编码乙醇脱氢酶）和THI3（编码脱羧酶）基因被删除。结果表明，通过单独删除ADH2或THI3基因的单基因删除突变体分别导致乙醛或杂醇的产量减少。在此期间，双基因缺失突变体酿酒酵母XF1-AT通过缺失构建ADH2和THI3同时进行。酿酒酵母XF1-AT的乙醇产量可以达到同等水平，但乙醛、异戊醇和异丁醇的产量分别降低了 42.09%、15.65% 和 20.16%。此外，在减少乙醛和杂醇的产生方面，ADH2缺失和THI3缺失之间没有相互作用。工程化的酿酒酵母XF1-AT 为酒精饮料酿造行业提供了一种减少乙醛和杂醇产量的新策略。