Studies behind mechanisms of Chlamydia trachomatis-induced recurrent spontaneous abortion is still in its infancy. Possible strategy for preventing recurrent spontaneous abortion at molecular level is needed. Despite its multifactorial aetiology, Chlamydia trachomatis is important cause of RSA. However, mechanism leading to RSA in C. trachomatis-positive patients is not understood and novel strategies are needed. It is hypothesized that microRNAs play important role in RSA regulation during infection. Study aimed to elucidate expression/role of urine-circulating miRs-320b, 221-3p, 146b-5p,-16,-24,-559 in recurrent spontaneous aborters with C. trachomatis infection and to find their target genes by bioinformatic analysis. First-void urine was collected from 30 non-pregnant women with RSA (Group I) and 30 non-pregnant women with ≥2 successful deliveries (Group II; Controls) attending Department of Obstetrics and Gynaecology, Vardhman Mahavir Medical College, Safdarjung hospital, New Delhi (India). PCR was performed to detect C. trachomatis. Expression of miRNAs was studied by quantitative real-time PCR while target genes/functional annotations were predicted by GO/KEGG databases. Data was statistically evaluated. 05 RSA patients were C. trachomatis-positive. Group I was subdivided into Group Ia (C. trachomatis-positive RSA; n = 5) and Group Ib (C. trachomatis-negative RSA; internal controls). miR-320b, -221-3p, −146b-5p, −16, −24 were significantly upregulated (miR-16 showed maximum 4.3 fold-change) while miR-559 was downregulated (0.5 fold-change) in Group Ia versus controls (‘p’<0.001). Bioinformatic analysis revealed that target genes of miRNAs in RSA are involved in apoptosis and AMPK signalling pathways. Results showed differential expression of miRNAs implyingmiR-16 and miR-559 as potential biomarkers of RSA in infected women. Furthermore, network of genes of differentially expressed miRNAs regulates RSA by targeting gene function in apoptosis, cell adhesion and angiogenesis.

沙眼衣原体诱发复发性自然流产机制的研究仍处于起步阶段。需要在分子水平上预防复发性自然流产的可能策略。尽管它的病因是多因素的，但沙眼衣原体是 RSA 的重要原因。然而，在沙眼衣原体阳性患者中导致 RSA 的机制尚不清楚，需要新的策略。假设 microRNA 在感染期间的 RSA 调节中起重要作用。旨在阐明尿循环 miRs-320b、221-3p、146b-5p、-16、-24、-559 在沙眼衣原体复发性自然流产患者中的表达/作用的研究感染并通过生物信息学分析找到它们的靶基因。从 30 名患有 RSA 的非孕妇（I 组）和 30 名 ≥ 2 次成功分娩（II 组；对照组）的非孕妇中收集了首次排尿，这些妇女在 Safdarjung 医院 Vardhman Mahavir 医学院妇产科就诊，新德里（印度）。进行PCR以检测沙眼衣原体。通过定量实时 PCR 研究 miRNA 的表达，而通过 GO/KEGG 数据库预测目标基因/功能注释。数据进行了统计评估。05 RSA 患者是沙眼衣原体阳性。I 组被细分为 Ia 组（沙眼衣原体阳性 RSA；n = 5）和 Ib 组（沙眼衣原体- 阴性 RSA；内部控制）。与对照组相比，Ia 组 miR-320b、-221-3p、-146b-5p、-16、-24 显着上调（miR-16 显示最大 4.3 倍变化），而 miR-559 下调（0.5 倍变化）（'p'<0.001）。生物信息学分析显示，RSA中miRNA的靶基因参与细胞凋亡和AMPK信号通路。结果显示 miRNA 的差异表达暗示 miR-16 和 miR-559 作为感染女性 RSA 的潜在生物标志物。此外，差异表达 miRNA 的基因网络通过靶向细胞凋亡、细胞粘附和血管生成中的基因功能来调节 RSA。