In this study, it was aimed to determine the antibiotic resistance of Escherichia coli strains isolated from samples taken from various children's parks of Ankara and to confirm the resistance by molecular methods. Five hundred fifty-four samples, including soil samples from 140 different parks and 414 swab samples from slides, swings, ferris wheels, seesaws, and other toys from 176 different parks, were taken. Fourty E. coli strains isolated from these samples were included in the study.

Antibiotic susceptibility tests of 40 E. coli isolates were performed by EUCAST recommendations. The resistance rates of E. coli isolates were found as ciprofloxacin 5%, ampicillin 17%, trimethoprim/sulfamethoxazole 15%, streptomycin 12.5%, tobramycin 5%, gentamicin 5%, cefotaxime 2.5%, and ceftazidime 2.5%. Intermediate rates were found as 95%, 90%, and 70% for tobramycin, gentamicin, and streptomycin respectively.

blaCTX-M β-lactamase gene was investigated for an isolate determined to be resistant to both cefotaxime and ceftazidime but blaCTXM gene could not be detected. Aminoglycoside resistance of strains has been investigated because of high intermediate sensitivity rates. For this purpose, aac(6′)-Ib, aac(3′)-IIa, aph(3′)-VI, ant(3′)-I, aac(3′)-IV, ant(2′)-Ia genes scanned, and were detected 97.5% of our isolates ant (3′)-I, %25 aac(6′)-Ib’, 5% aac(3′)-IIa, 2.5% ant(2′)-Ia. Also, aph(3′)-VI, and aac(3′)-IV genes could not be detected in any of the isolates. Consequently, it has been revealed that resistant E. coli strains isolated from children's parks can pose a potential risk in public health for transmission of resistant genes.

本研究旨在确定从安卡拉各个儿童公园采集的样本中分离的大肠杆菌菌株的抗生素耐药性，并通过分子方法确认耐药性。采集了 554 个样品，包括来自 140 个不同公园的土壤样品和来自 176 个不同公园的滑梯、秋千、摩天轮、跷跷板和其他玩具的 414 个拭子样品。从这些样品中分离出的 40 株大肠杆菌被纳入研究。

EUCAST 建议对 40 种大肠杆菌分离株进行了抗生素敏感性测试。大肠杆菌分离株的耐药率为环丙沙星 5%、氨苄西林 17%、甲氧苄啶/磺胺甲恶唑 15%、链霉素 12.5%、妥布霉素 5%、庆大霉素 5%、头孢噻肟 2.5% 和头孢他啶 2.5%。妥布霉素、庆大霉素和链霉素的中间率分别为 95%、90% 和 70%。

对 blaCTX-M β-内酰胺酶基因进行了研究，以确定对头孢噻肟和头孢他啶均具有抗性的分离株，但无法检测到 blaCTXM 基因。由于较高的中间敏感率，已经对菌株的氨基糖苷类抗性进行了研究。为此，aac(6')-Ib、aac(3')-IIa、aph(3')-VI、ant(3')-I、aac(3')-IV、ant(2')-扫描 Ia 基因，并检测到我们分离株 ant (3')-I、%25 aac(6')-Ib'、5% aac(3')-IIa、2.5% ant(2')-Ia 的 97.5% . 此外，在任何分离株中均未检测到 aph(3')-VI 和 aac(3')-IV 基因。因此，研究表明，从儿童公园中分离出的耐药大肠杆菌菌株可能会对公共健康造成潜在的耐药基因传播风险。