Non-destructive methodologies based on the use of artificial substrates (AS) for quantitative sampling of macrofauna have been used to solve sampling problems in complex benthic environment, such as rocky reefs. The macrofauna assemblages of two different types of AS (dendritic and crevice), at two different locations were studied. The main goal was to evaluate the complementarity of dendritic and crevice AS when sampling the macrofauna associated with rocky environments, in two scenarios: within the same location and between locations. With this approach, we intend to contribute to the development of a non-destructive sampling methodology based on AS. The hypotheses tested were that i) the assemblages associated with each type of AS would differ between them and, ii) regardless of AS type, assemblages would differ between locations. Our results revealed significant differences after three months between the macrofauna assemblages from dendritic and crevice substrates in both locations and differences between locations irrespective of the AS type used. Hence, due to the high number of species that only were attracted by each type of AS and the species settlement preferences, our findings have shown the complementarity of the two types of AS (dendritic and crevice). This suggests that the mixed use of AS could be the best approach to non-destructive standard monitoring programs based on benthic marine macrofauna.

基于使用人工基质 (AS) 对大型动物进行定量采样的非破坏性方法已被用于解决复杂底栖环境（如岩礁）中的采样问题。研究了两个不同位置的两种不同类型 AS（树突状和裂缝）的大型动物群。主要目标是在两种情况下对与岩石环境相关的大型动物进行采样时评估树突和裂缝 AS 的互补性：在同一位置内和位置之间。通过这种方法，我们打算为基于 AS 的无损采样方法的开发做出贡献。测试的假设是 i) 与每种类型的 AS 相关的组合在它们之间会有所不同，ii) 无论 AS 类型如何，组合在位置之间都会有所不同。我们的结果显示，三个月后来自两个位置的树突和裂缝基质的大型动物群之间存在显着差异，并且无论使用的 AS 类型如何，位置之间的差异都存在。因此，由于大量物种仅被每种类型的 AS 和物种定居偏好所吸引，我们的研究结果显示了两种类型的 AS（树突状和裂缝）的互补性。这表明混合使用 AS 可能是基于底栖海洋大型动物的无损标准监测计划的最佳方法。由于大量物种仅被每种类型的 AS 和物种定居偏好所吸引，我们的研究结果显示了两种类型的 AS（树突状和裂缝）的互补性。这表明混合使用 AS 可能是基于底栖海洋大型动物的无损标准监测计划的最佳方法。由于大量物种仅被每种类型的 AS 和物种定居偏好所吸引，我们的研究结果显示了两种类型的 AS（树突状和裂缝）的互补性。这表明混合使用 AS 可能是基于底栖海洋大型动物的无损标准监测计划的最佳方法。