It has been reported that circ-ATAD1 promotes the development of gastric cancer. This study was carried out to analyze the role of circ-ATAD1 in endometrial cancer (EC). EC and paired non-tumor tissues from EC patients (n = 60) were subjected to the isolation of total RNA and RT-qPCR to analyze the expression of circ-ATAD1 and miR-10a. Correlations between circ-ATAD1 and miR-10a were analyzed by Pearson’s correlation coefficient. The effects of circ-ATAD1 overexpression on miR-10a expression and methylation were analyzed by RT-qPCR and methylation-specific PCR (MSP). The roles of circ-ATAD1 and miR-10a in regulating EC cell invasion and migration were analyzed by Transwell assays. We found that circ-ATAD1 was downregulated in EC, while miR-10a was upregulated in EC. An inverse correlation between circ-ATAD1 and miR-10a was observed across EC samples. In EC cells, circ-ATAD1 overexpression decreased miR-10a expression and increased miR-10a gene methylation. Transwell assay analysis showed that circ-ATAD1 overexpression totally reversed the enhancing effects of miR-10a on EC cell invasion and migration. Circ-ATAD1 is downregulated in EC and may suppress EC cell invasion and migration by downregulating miR-10a through methylation.

据报道，circ-ATAD1促进胃癌的发展。本研究旨在分析 circ-ATAD1 在子宫内膜癌 (EC) 中的作用。EC 和来自 EC 患者的配对非肿瘤组织 ( n = 60) 进行总 RNA 的分离和 RT-qPCR 以分析 circ-ATAD1 和 miR-10a 的表达。通过 Pearson 相关系数分析 circ-ATAD1 和 miR-10a 之间的相关性。通过RT-qPCR和甲基化特异性PCR（MSP）分析circ-ATAD1过表达对miR-10a表达和甲基化的影响。通过 Transwell 测定分析了 circ-ATAD1 和 miR-10a 在调节 EC 细胞侵袭和迁移中的作用。我们发现 circ-ATAD1 在 EC 中下调，而 miR-10a 在 EC 中上调。在 EC 样本中观察到 circ-ATAD1 和 miR-10a 之间的负相关。在 EC 细胞中，circ-ATAD1 过表达降低了 miR-10a 表达并增加了 miR-10a 基因甲基化。Transwell 分析表明，circ-ATAD1 过表达完全逆转了 miR-10a 对 EC 细胞侵袭和迁移的增强作用。Circ-ATAD1 在 EC 中下调，可能通过甲基化下调 miR-10a 来抑制 EC 细胞侵袭和迁移。