Wound management is essential to provide an appropriate environment for healing or avoiding wound or postsurgery contamination, which remains a serious clinical issue. In this study, we developed a novel wound dressing by using the electrospinning technique to draw continuous polymeric fibers from either a polymer solution or polymer melt to fabricate wound dressings with good medicinal properties. Polycaprolactone (PCL) is a biomaterial with good properties that was utilized in this work to produce PCL electrospun mats. Given their hydrophobic properties, however, PCL electrospun mats were hydrolyzed by alkali hydrolysis to permit antibacterial agent attachment. To confer antibacterial properties, pexiganan, an antimicrobial peptide (AMP), was employed as the active agent at various concentrations. The results indicate that PCL electrospun mats treated with pexiganan exhibited efficient bacterial inhibition of both gram-positive and gram-negative bacteria. To assess in vitro cytotoxicity, the viability of human dermal fibroblast (HDF) cells following application of the treated mats was measured and found to be significantly decreased at 1 day depending on the amount of deposited agent; nevertheless, the PCL electrospun mats were validated according to ISO 10993–5:2009.

伤口管理对于为愈合或避免伤口或术后污染提供适当的环境至关重要，这仍然是一个严重的临床问题。在这项研究中，我们开发了一种新型伤口敷料，通过使用静电纺丝技术从聚合物溶液或聚合物熔体中提取连续的聚合物纤维来制造具有良好药用特性的伤口敷料。聚己内酯 (PCL) 是一种具有良好性能的生物材料，在这项工作中用于生产 PCL 电纺垫。然而，鉴于它们的疏水特性，PCL 电纺垫通过碱水解进行水解，以允许抗菌剂附着。为了赋予抗菌特性，将一种抗菌肽 (AMP) pexiganan 用作各种浓度的活性剂。结果表明，用 pexiganan 处理的 PCL 电纺垫表现出对革兰氏阳性菌和革兰氏阴性菌的有效细菌抑制。评估体外细胞毒性，测量了施用处理过的垫后人真皮成纤维细胞 (HDF) 细胞的活力，发现其在 1 天时显着降低，这取决于沉积剂的量；尽管如此，PCL 电纺垫还是根据 ISO 10993-5:2009 进行了验证。