Postharvest loss is a major constraint in the production of grapes, both in terms of quality and quantity. In this study, 33 yeasts were isolated from the fructosphere of grapes and tested for their antifungal activity against Aspergillus spp., which infects grapes during the postharvest stage. The results revealed that the yeast isolate YBB3 exhibited maximum inhibitory effect against Aspergillus spp. in dual plate assay (57% over control). Molecular characterization of YBB3 showed 99.31% identity with Hanseniaspora guilliermondii. Wound site colonization of H. guilliermondii in grape cv. Thompson Seedless showed 80% inhibition of Aspergillus spp. over the control in vitro. YBB3 produced volatile, non-volatile and thermostable compounds, which inhibited the mycelial growth of Aspergillus spp. In addition, YBB3 produced killer toxins, which were identified by the presence of blue colour-stained cells. Moreover, glycerol-based liquid bioformulations of H. guilliermondii (YBB3) showed maximum inhibitory effect against Aspergillus spp. (96%) over the control on inoculated wounded grapes berries. Thus, the yeast isolate H. guilliermondii identified and characterized in this study is worthy of further studies for the sustainable management of Aspergillus rot in grapes.

收获后损失是葡萄生产的主要制约因素，无论是质量还是数量。在这项研究中，从葡萄果圈中分离出 33 种酵母，并测试了它们对曲霉属的抗真菌活性，曲霉属在收获后阶段感染葡萄。结果表明，酵母分离物 YBB3 对曲霉属表现出最大的抑制作用。在双板测定中（超过对照 57%）。YBB3 的分子特征表明与Hanseniaspora guilliermondii具有 99.31% 的同一性。H. guilliermondii在葡萄品种中的伤口定植。Thompson Seedless 对曲霉菌有 80% 的抑制作用属 过体外对照。YBB3 产生挥发性、非挥发性和热稳定的化合物，抑制曲霉属的菌丝体生长。此外，YBB3 产生杀伤性毒素，通过蓝色染色细胞的存在来鉴定。此外，基于甘油的H. guilliermondii (YBB3)液体生物制剂对曲霉属表现出最大的抑制作用。(96%) 超过对接种受伤葡萄浆果的控制。因此，本研究中鉴定和表征的酵母分离株H. guilliermondii值得进一步研究葡萄中的曲霉腐烂的可持续管理。