The outbreak of new viral strains promotes advances in universal diagnostic techniques for detecting infectious diseases with unknown viral sequence. Long double-stranded RNA (dsRNA), a hallmark of infections, serves as a virus marker for prompt detection of viruses with unknown genomes. Here, we report on-chip paper electrophoresis for ultrafast screening of infectious diseases. Negatively charged RNAs pass through the micro and nanoscale pores of cellulose in order of size under an external electric field applied to the paper microfluidic channel. Quantitative separation of long dsRNA mimicking poly I:C was analyzed from 1.67 to 33 ng·μL⁻¹, which is close to the viral dsRNA concentration in infected cells. This paper-based capillary electrophoresis chip (paper CE chip) can provide a new diagnostic platform for ultrafast viral disease detection at the point-of-care (POC) level.

新病毒株的爆发促进了用于检测病毒序列未知的传染病的通用诊断技术的进步。长双链 RNA (dsRNA) 是感染的标志，可作为病毒标记，用于迅速检测基因组未知的病毒。在这里，我们报告了用于传染病超快速筛查的片上纸电泳。在施加到纸微流体通道的外部电场下，带负电荷的 RNA 按大小顺序穿过纤维素的微孔和纳米孔。模拟poly I:C的长dsRNA的定量分离分析为1.67至33 ng·μL ^-1，这接近感染细胞中的病毒 dsRNA 浓度。这种纸基毛细管电泳芯片（纸CE芯片）可以为即时（POC）级别的超快速病毒性疾病检测提供新的诊断平台。