Titanium dioxide nanoparticles (TiO₂ NPs) can potentially impact constructed wetlands (CWs). The aim of this study was to investigate the rapid impacts on nitrogen removal performance and on the microbial community after short-term exposure to TiO₂ NPs in CWs, which were determined via 16S rDNA amplicon sequencing and Biolog-Eco technologies. The results showed that removal efficiencies declined by 23.9%, 32.2%, and 38.8% for ammonia nitrogen (NH₄⁺-N) and by 26.5%, 43.4%, and 71.4% for nitrate nitrogen at TiO₂ NP concentrations of 50, 100, and 150 mg/L, respectively. Bacterial damage and TiO₂ NP agglomeration on the packing increased as the TiO₂ NP concentration increased. Microbes showed different tolerances against TiO₂ NPs, and members, such as the bacteria removing organic pollutants and NH₄⁺-N, were susceptible to TiO₂ NPs. The order of microbial utilisation of the six carbon sources was determined, and the utilisation ratios declined as TiO₂ NP concentration increased. The concentration of 50 mg/L TiO₂ NPs impaired the functions of nitrifiers and denitrifiers in CWs after short-term exposure, and higher concentrations (100 mg/L and 200 mg/L) had no additional negative impact on the packing compared with the concentration of 50 mg/L.

二氧化钛纳米粒子 (TiO ₂ NPs) 可能会影响人工湿地 (CWs)。本研究的目的是调查短期暴露于 CW 中的TiO ₂ NPs后对脱氮性能和微生物群落的快速影响，这是通过 16S rDNA 扩增子测序和 Biolog-Eco 技术确定的。结果表明，在 TiO ₂ NP 浓度为 50、100 时，氨氮 (NH ₄ ⁺ -N) 的去除效率分别下降了 23.9%、32.2% 和 38.8%，硝态氮的去除效率下降了26.5%、43.4% 和 71.4%。和 150 毫克/升，分别。填料上的细菌损伤和 TiO ₂ NP 团聚随着 TiO _{2 的}增加而增加NP 浓度增加。微生物对TiO ₂ NPs表现出不同的耐受性，去除有机污染物的细菌和NH ₄ ⁺ -N 等成员对TiO ₂ NPs敏感。确定了六种碳源的微生物利用顺序，利用率随着TiO ₂ NP浓度的增加而下降。短期暴露后，50 mg/L TiO ₂ NPs的浓度会削弱 CWs 中硝化剂和反硝化剂的功能，并且更高的浓度（100 mg/L 和 200 mg/L）对填料没有额外的负面影响。浓度为 50 毫克/升。