Biological nitrogen and phosphorus removal often requires an external carbon source to be effective during wastewater treatments. The purpose of this work was to evaluate a sequencing batch reactor (SBR) operated in a non-conventional anoxic/aerated (An/O) regime (alternated condition) treating synthetic wastewater simulating secondary anaerobic effluent mainly composed by P-phosphate and N-nitrate. The focus was to report the achieved biological efficiency removal of phosphorus and nitrate from the synthetic inlet when adding pre-fermented glycerol as the sole external carbon source. Experimental phase I aimed at the glycerol fermentation in batch reactors, leading to the formation of ethanol and volatile fatty acids. During experimental phase II, a non-acclimated (C1) and an acclimated biomass (C2) were used as inoculum of the SBR operated in the (An/O) regime. The anoxic phase was set to couple denitrification and P-release/uptake. The aerated phase was set to remove any remaining phosphorus. The fermented glycerol was applied in 15 gCOD g⁻¹ N-NO₃⁻ and 29 gCOD g⁻¹P-PO₄⁻³ ratios in a sequential batch reactor during a non-aerated phase. This operation favored the development and maintenance of phosphorus-accumulating organisms (PAO), as well as denitrifying organisms, resulting in approximately 90 ± 11% and 98 ± 3% for phosphorus and nitrogen removal, respectively. The study showed that the fermented glycerol can be successfully applied as an electron donor for biological nitrogen and phosphorus removal during wastewater treatments.

生物脱氮除磷通常需要外部碳源才能在废水处理过程中有效。这项工作的目的是评估在非常规缺氧/曝气 (An/O) 制度（交替条件）下运行的序批式反应器 (SBR) 处理合成废水，模拟主要由 P-磷酸盐和 N- 组成的二次厌氧流出物。硝酸盐。重点是报告当添加预发酵甘油作为唯一的外部碳源时，从合成入口去除磷和硝酸盐所达到的生物效率。实验阶段 I 旨在分批反应器中的甘油发酵，导致乙醇和挥发性脂肪酸的形成。在第二阶段的实验中，未驯化的 (C1) 和驯化的生物质 (C2) 用作在 (An/O) 制度下运行的 SBR 的接种物。缺氧阶段设置为耦合反硝化和磷释放/吸收。充气阶段被设置为去除任何剩余的磷。发酵甘油的用量为 15 gCOD g^-1  N-NO ₃ ^-和29 gCOD g ^-1 P-PO ₄ ^-3比率在非充气阶段的顺序间歇反应器中。该操作有利于积磷生物 (PAO) 以及反硝化生物的发育和维持，分别产生约 90 ± 11% 和 98 ± 3% 的磷和氮去除率。研究表明，发酵甘油可以成功地作为电子供体用于废水处理过程中的生物脱氮除磷。