Recently, green nanotechnology is considered a more suitable and safer tool for medical applications due to its nature reductants with low toxicity and low eco-hazard. The micro-algal biomass was used for the nanoparticles biosynthesis, depending on using whole algal cell cultivation. During the current study, cyanobacteria Oscillatoria sp. and Spirulina platensis exhibited their ability to synthesize silver oxide (Ag₂O|AgO-NPs) and gold nanoparticles (Au-NPs), respectively. The characterization results confirmed the formation of Oscillatoria sp.-mediated silver oxide nanoparticles (O.Ag₂O|AgO-NPs) and S. platensis-mediated gold nanoparticles (S.Au-NPs). Three different methods had been examined, (i) culture-free cells (secondary metabolites), (ii) algal aqueous extract, and (iii) whole cells cultivation (in vivo). UV–Vis spectroscopy confirmed the biosynthesized O.Ag-NPs at 438 nm, while S.Au-NPs reached 545 nm by whole cells cultivation technique. TEM scanning indicated the formation of spherical-shaped O.Ag₂O|AgO-NPs with an average size ranged between 10.49 and 45.81 nm. S.Au-NPs also were detected in triangular, pentagonal, and slightly spherical shapes with an average size of 15.49–55.08 nm. Both O.Ag₂O|AgO-NPs, and S.Au-NPs demonstrated antibacterial and antifungal properties against Gram-positive and Gram-negative bacteria. S.Au-NPs were more effective than O.Ag₂O|AgO-NPs, which recorded high significant MIC results against Bacillus subtilis ATCC 19659 (1.95 μg/ml), Salmonella typhi ATCC14028 (3.90 μg/ml), and Candida tropicalis ATCC 1380 (1.1 μg/ml) after 24 h treatment, comparing with the control. It is concluded that O.Ag₂O|AgO-NPs and S.Au-NPs have efficient antibacterial and antifungal activities.

近年来，绿色纳米技术因其具有低毒性和低生态危害的天然还原剂而被认为是更适合和更安全的医疗应用工具。微藻生物质用于纳米颗粒生物合成，这取决于使用全藻细胞培养。在目前的研究中，蓝藻Oscillatoria sp。Spirulina platensis和Spirulina platensis 分别表现出合成氧化银 (Ag ₂ O|AgO-NPs) 和金纳米粒子 (Au-NPs) 的能力。表征结果证实了Oscillatoria sp. 介导的氧化银纳米粒子 ( O .Ag ₂ O|AgO-NPs) 和S.platensis介导的金纳米粒子的形成 (S. Au-NPs）。已经检查了三种不同的方法，（i）无培养细胞（次级代谢物），（ii）藻类水提取物，和（iii）全细胞培养（体内）。UV-Vis 光谱证实了生物合成的O. Ag-NPs 在 438 nm，而S. Au-NPs 通过全细胞培养技术达到了 545 nm。TEM 扫描表明形成了球形O .Ag ₂ O|AgO-NPs，平均尺寸介于 10.49 和 45.81 nm 之间。S. Au-NPs 也被检测到三角形、五边形和略呈球形的形状，平均尺寸为 15.49-55.08 nm。既O.的Ag ₂ ö| AGO-的NP，和小号.Au-NPs 显示出对革兰氏阳性和革兰氏阴性细菌的抗菌和抗真菌特性。S .Au-NPs 比O .Ag ₂ O|AgO-NPs更有效，后者记录了对枯草芽孢杆菌ATCC 19659 (1.95 μg/ml)、伤寒沙门氏菌ATCC14028 (3.90 μg/ml) 和热带念珠菌的高显着 MIC 结果与对照相比，处理 24 小时后的 ATCC 1380 (1.1 μg/ml)。结论是O .Ag ₂ O|AgO-NPs 和S. Au-NPs 具有有效的抗菌和抗真菌活性。