Aflatoxin B1 (AFB1) contamination in feed and food seriously threatens the healthy growth of animals and humans, and it may lead to huge economic losses in livestock and poultry production. Therefore, screening of high-efficient AFB1-degrading bacteria is necessary to ensure the safety of feed and food. The study aims to isolate and characterize bacteria from various sources to explore its AFB1 degradation potential. Fifteen bacterial were obtained using a medium containing coumarin as the sole carbon source; only one strain showed a good-degrading ability in culture media by adding AFB1 and it was selected for further studies. A gram-negative and spore-forming, designated E1, was identified as Paenibacillus pabuli, with the highest sequence similarity to P. pabuli NBRC13638T (98.97%). The growth of the strain E1 was observed under 22-47 °C, pH 5.5-9.5 and NaCl concentration 0-6% (w/v), with optimum growth at 37 °C, pH 7.5 and 1% NaCl. The biodegradation characteristics of object strain were detected by high performance liquid chromatography (HPLC). The degradation ratio of AFB1 reached 55% at 24 h and 70.2% at 48 h. After 96 h, the degradation rate of AFB1 reached 85.9%. The active degradation components were present in the cell-free supernatant of strain E1, and the degradation ratio of AFB1 reached 80.0% after 96 h. It is the first report that genus Paenibacillus could degrade AFB1. Moreover, E1 has highly adaptable to diverse environmental conditions. It will be a potential candidate for biodegradation of mycotoxins in feed and food.

中文翻译：

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分离、鉴定和表征 Paenibacillus pabuli E1 以探索其黄曲霉毒素 B1 降解潜力。

饲料和食品中的黄曲霉毒素B1（AFB1）污染严重威胁着动物和人类的健康生长，可能给畜禽生产带来巨大的经济损失。因此，筛选高效降解 AFB1 的细菌是保证饲料和食品安全的必要条件。该研究旨在从各种来源分离和表征细菌，以探索其 AFB1 降解潜力。使用含有香豆素作为唯一碳源的培养基获得了 15 种细菌；只有一种菌株通过添加AFB1在培养基中表现出良好的降解能力，并被选择用于进一步研究。革兰氏阴性和孢子形成，命名为 E1，被鉴定为 Paenibacillus pabuli，与 P. pabuli NBRC13638T (98.97%) 具有最高的序列相似性。在 22-47 °C、pH 5 下观察到菌株 E1 的生长。5-9.5 和 NaCl 浓度 0-6% (w/v)，在 37 °C、pH 7.5 和 1% NaCl 下最佳生长。采用高效液相色谱法(HPLC)检测目标菌株的生物降解特性。AFB1 的降解率在 24 h 时达到 55%，在 48 h 时达到 70.2%。96 h后，AFB1的降解率达到85.9%。菌株E1的无细胞上清液中存在活性降解成分，96 h后AFB1的降解率达到80.0%。这是首次报道类芽孢杆菌属能够降解AFB1。此外，E1 对各种环境条件具有很强的适应性。它将成为生物降解饲料和食品中真菌毒素的潜在候选者。采用高效液相色谱法(HPLC)检测目标菌株的生物降解特性。AFB1 的降解率在 24 h 时达到 55%，在 48 h 时达到 70.2%。96 h后，AFB1的降解率达到85.9%。菌株E1的无细胞上清液中存在活性降解成分，96 h后AFB1的降解率达到80.0%。这是首次报道类芽孢杆菌属能够降解AFB1。此外，E1 对各种环境条件具有很强的适应性。它将成为生物降解饲料和食品中真菌毒素的潜在候选者。采用高效液相色谱法(HPLC)检测目标菌株的生物降解特性。AFB1 的降解率在 24 h 时达到 55%，在 48 h 时达到 70.2%。96 h后，AFB1的降解率达到85.9%。菌株E1的无细胞上清液中存在活性降解成分，96 h后AFB1的降解率达到80.0%。这是首次报道类芽孢杆菌属能够降解AFB1。此外，E1 对各种环境条件具有很强的适应性。它将成为生物降解饲料和食品中真菌毒素的潜在候选者。菌株E1的无细胞上清液中存在活性降解成分，96 h后AFB1的降解率达到80.0%。这是首次报道类芽孢杆菌属能够降解AFB1。此外，E1 对各种环境条件具有很强的适应性。它将成为生物降解饲料和食品中真菌毒素的潜在候选者。菌株E1的无细胞上清液中存在活性降解成分，96 h后AFB1的降解率达到80.0%。这是首次报道类芽孢杆菌属能够降解AFB1。此外，E1 对各种环境条件具有很强的适应性。它将成为生物降解饲料和食品中真菌毒素的潜在候选者。