The methane-mitigating potency of cashew nutshell liquid (CNSL) was evaluated by investigating gas production from batch cultures using feces from Thai native ruminants that had been incubated for different periods. Feces was obtained from four Thai native cattle and four swamp buffaloes reared under practical feeding conditions at the Kasetsart University farm, Thailand. Fecal slurry from the same farm was also included in the analysis. CNSL addition successfully suppressed the methane production potential of feces from both ruminants by shifting short chain fatty acid profiles towards propionate production. Methane mitigation continued for almost 150 days, although the degree of mitigation was more apparent from Day 0 to Day 30. Bacterial and archaeal community shifts with CNSL addition were observed in feces from both ruminants; specifically, Bacteroides increased, whereas Lachnospiraceae and Ruminococcaceae decreased in feces to which CNSL was added. Fecal slurry did not show marked changes in gas production with CNSL addition. The findings showed that the addition of CNSL to the feces of ruminants native to the Southeast Asian region can suppress methane emission. Because CNSL can be easily obtained as a byproduct of the local cashew industry in this region, its on-site application might be ideal.

中文翻译：

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腰果壳液可能通过改变粪便微生物群来减轻泰国本土反刍家畜粪便中的甲烷排放

腰果壳液 (CNSL) 的甲烷缓解效力是通过研究使用经过不同时期孵化的泰国本地反刍动物的粪便进行分批培养的气体产量来评估的。粪便取自泰国 Kasetsart 大学农场在实际饲养条件下饲养的四头泰国本地牛和四头沼泽水牛。来自同一农场的粪便泥浆也包括在分析中。添加 CNSL 通过将短链脂肪酸谱转向丙酸盐生产，成功地抑制了两种反刍动物粪便中甲烷的产生潜力。尽管从第 0 天到第 30 天缓解程度更加明显，但甲烷缓解持续了将近 150 天。在两种反刍动物的粪便中观察到添加 CNSL 后细菌和古细菌群落发生了变化；具体来说，添加 CNSL 的粪便中拟杆菌增加，而毛螺菌科和瘤胃球菌科减少。添加 CNSL 后，粪便浆液的产气量没有显着变化。研究结果表明，在原产于东南亚地区的反刍动物的粪便中添加 CNSL 可以抑制甲烷排放。由于 CNSL 作为该地区当地腰果产业的副产品很容易获得，因此现场应用可能是理想的选择。