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Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats
The Journal of Veterinary Diagnostic Investigation ( IF 1.2 ) Pub Date : 2021-08-17 , DOI: 10.1177/10406387211038918
Elisabeth Neubert 1 , Karin Weber 1
Affiliation  

We analyzed urine samples from 191 cats for bacteriuria with an automated urine sediment analyzer (Idexx SediVue Dx), combined with image review by an observer, and compared to bacteriologic culture results. Sixty-nine samples were unambiguously assigned to be free of bacteria by the instrument and the observer, and no bacterial growth was detected. Twenty-seven samples were unambiguously assigned to have bacteriuria; 24 of these 27 samples were culture-positive. For these samples, bacteriuria was predicted with a sensitivity of 100% and a specificity of 96%. A clear assignment was not possible for 95 samples, 81 of which were culture-negative. Specificity dropped to 45% when all samples were considered. Using the automated leukocyte count to predict bacteriuria, sensitivity was 82% and specificity was 75%. Automated sediment analysis is faster and less observer-dependent than sediment analysis under a microscope, but accurate detection of bacteriuria remains difficult in a large proportion of samples. Bacteriuria was significantly associated with leukocyte count; the leukocyte count was >5/high power field in 82% of culture-positive samples.



中文翻译:

使用 Idexx SediVue Dx 预测猫尿细菌培养的需要

我们使用自动尿沉渣分析仪 (Idexx SediVue Dx) 分析了 191 只猫的尿样,并结合观察者的图像审查,并与细菌培养结果进行了比较。仪器和观察者明确将 69 个样品指定为不含细菌,并且未检测到细菌生长。27 个样本被明确指定为有菌尿;这 27 个样本中有 24 个为培养阳性。对于这些样本,菌尿的预测灵敏度为 100%,特异性为 96%。无法对 95 个样本进行明确分配,其中 81 个样本为培养阴性。当考虑所有样品时,特异性下降到 45%。使用自动白细胞计数来预测菌尿,敏感性为 82%,特异性为 75%。与显微镜下的沉积物分析相比,自动沉积物分析速度更快,对观察者的依赖性更小,但在大部分样品中准确检测菌尿仍然很困难。菌尿与白细胞计数显着相关;在 82% 的培养阳性样本中,白细胞计数 >5/高倍视野。

更新日期:2021-08-19
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