ABSTRACT

Diazinon is an organophosphorus pesticide, which may have potential toxic effects on the liver and immune system; however, the underlying mechanisms remain mostly unidentified. This work is aimed at evaluating the oxidative stress and cell cycle alterations elicited by low-dose diazinon in a rat liver cell line (BRL-3A) and spleen mononuclear cells (SMC) from Wistar rats. Diazinon (10–50 μM) caused early reactive oxygen species (ROS) generation (from 4 h) as well as increased O₂^•- level (from 0.5 h), which led to subsequent lipid peroxidation at 24 h, in BRL-3A cells. In SMC, diazinon (20 μM) produced similar increases in ROS levels, at 4 and 24 h, with the highest O₂^•- level being found at 4 h. Low-dose diazinon induced G1-phase arrest and cell death in hepatic cells and SMC. Therefore, diazinon could affect the liver and the immunological system through the premature oxidative stress induction.

Abbreviations: O₂^•-: superoxide anion radical; ROS: reactive oxygen species; SMC: spleen mononuclear cells; TBARS: thiobarbituric acid reactive substances

摘要

二嗪农是一种有机磷农药，可能对肝脏和免疫系统有潜在的毒性作用；然而，潜在的机制大多仍未确定。这项工作旨在评估低剂量二嗪农在大鼠肝细胞系 (BRL-3A) 和来自 Wistar 大鼠的脾单核细胞 (SMC) 中引起的氧化应激和细胞周期改变。二嗪农 (10–50 μM) 在 BRL-3A 中引起早期活性氧 (ROS) 生成（从 4 小时开始）以及增加 O ₂ ^•-水平（从 0.5 小时开始），这导致随后的 24 小时脂质过氧化细胞。在 SMC 中，二嗪农 (20 μM) 在 4 小时和 24 小时时产生了类似的 ROS 水平增加，其中 O ₂ ^•--在 4 小时发现水平。低剂量二嗪农在肝细胞和 SMC 中诱导 G1 期阻滞和细胞死亡。因此，二嗪农可通过过早的氧化应激诱导影响肝脏和免疫系统。

缩写： O ₂ ^•-：超氧阴离子自由基；ROS：活性氧；SMC：脾单核细胞；TBARS：硫代巴比妥酸反应物质