Rolling circle amplification (RCA) is a powerful tool for the construction of DNA nanomaterials such as hydrogels, high-performance scaffolds and DNA nanoflowers (DNFs), hybrid materials formed of DNA and magnesium pyrophosphate. Such DNA nanomaterials have great potential in therapeutics, imaging, protein immobilisation, and drug delivery, yet limited chemistry is available to expand their functionality. Here, we present orthogonal strategies to produce densely modified RCA products and DNFs. We provide methods to selectively modify the DNA component and/or the protein cargo of these materials, thereby greatly expanding the range of chemical functionalities available to these systems. We have used our methodology to construct DNFs bearing multiple surface aptamers and peptides capable of binding to cancer cells that overexpress the HER2 oncobiomarker, demonstrating their potential for diagnostic and therapeutic applications.

中文翻译：

---

扩展滚环扩增产生的 DNA 纳米材料的化学功能


滚环扩增 (RCA) 是构建 DNA 纳米材料的强大工具，例如水凝胶、高性能支架和 DNA 纳米花 (DNF)、DNA 和焦磷酸镁形成的混合材料。这种 DNA 纳米材料在治疗、成像、蛋白质固定和药物输送方面具有巨大潜力，但可用于扩展其功能的化学物质有限。在这里，我们提出了生产密集修饰的 RCA 产品和 DNF 的正交策略。我们提供选择性修饰这些材料的 DNA 成分和/或蛋白质货物的方法，从而极大地扩展了这些系统可用的化学功能范围。我们使用我们的方法构建了带有多个表面适体和肽的 DNF，这些表面适体和肽能够与过度表达 HER2 肿瘤生物标志物的癌细胞结合，证明了它们在诊断和治疗应用中的潜力。