This experiment was designed to investigate the effects of mineral element imbalance on gene transcription in peripheral circulating neutrophils of sheep. Ten female sheep weighing 30 kg of similar age and physiological condition were randomly selected and injected via central venous catheterization with EDTA at a concentration of 4% (W/V) to construct a model of mineral element imbalance. As self-control, the sheep were divided into control [Con, before EDTA injection, time point 0 (T0)], EDTA [12 h after EDTA injection, time point 12 h (T12h)] and recovery [HF, 7 days after injection, time point 7 days (T7d)] groups. Whole blood was collected, and serum and neutrophils were separated for ionomic and transcriptomic analysis. The results showed that levels of P (P < 0.05), Zn (P < 0.01), K (P < 0.01) and some other elements were significantly lower in sheep in the EDTA group than in those in the control group, but levels of P (P > 0.05), Zn (P > 0.05) and K (P > 0.05) were similar in the recovery and control groups. Levels of Ca (P > 0.05) and Cu (P < 0.05) were higher after injection, and levels of Cu (P < 0.01) continued to increase during the recovery period. There were 1561 genes, including S100 family genes, significantly differentially expressed in neutrophils between the control and trial groups, and these genes were mainly enriched in the categories defense response, immune response, regulation of interleukin production and other functions. The top enriched signaling pathways were phagosome, NF-κB and Toll-like receptor. Mineral element homeostasis imbalance affected the gene transcriptome of circulating neutrophils, demonstrating the importance of carefully controlling the addition of mineral elements.

本实验旨在研究矿物质元素失衡对绵羊外周循环中性粒细胞基因转录的影响。随机选取10只体重30公斤、年龄和生理状况相近的母羊，通过中心静脉导管注射浓度为4%（W/V）的EDTA，构建矿质元素失衡模型。作为自我对照，将绵羊分为对照[Con，EDTA注射前，时间点0（T0）]，EDTA[EDTA注射后12 h，时间点12 h（T12h）]和恢复[HF，7天后注射，时间点7天（T7d）]组。收集全血，分离血清和中性粒细胞进行离子组学和转录组学分析。结果表明，P ( P  < 0.05)、Zn ( P < 0.01)、K( P  < 0.01)等元素含量EDTA组羊明显低于对照组，但P( P  > 0.05)、Zn( P  > 0.05)和K ( P  > 0.05）在恢复组和对照组中相似。注射后Ca ( P  > 0.05) 和 Cu ( P  < 0.05) 水平较高，而 Cu ( P < 0.01) 在恢复期间继续增加。包括S100家族基因在内的1561个基因在对照组和试验组的中性粒细胞中存在显着差异表达，这些基因主要富集在防御反应、免疫反应、调节白介素产生等功能。最丰富的信号通路是吞噬体、NF-κB 和 Toll 样受体。矿物质元素稳态失衡影响循环中性粒细胞的基因转录组，证明了谨慎控制矿物质元素添加的重要性。