High-density lipoprotein (HDL) particles comprising heterogeneous subclasses of different functions exert anti-inflammatory effects by interacting with immune-response cells. However, the relationship of HDL subclasses with immune-response cells in metabolic unhealth/obesity has not been defined clearly. The purpose of this study was to delineate the relational changes of HDL subclasses with immune cells and inflammatory markers in metabolic unhealth/obesity to understand the role of anti-inflammatory HDL subclasses. A total of 316 participants were classified by metabolic health. HDL subclasses were detected by microfluidic chip electrophoresis. White blood cell (WBC) counts and lymphocytes were assessed using automatic haematology analyser. Levels of high-sensitivity C-reactive protein (hs-CRP) and interleukin 6 (IL-6) were measured. In our study, not only the distribution of HDL subclasses, but also HDL-related structural proteins changed with the deterioration of metabolic disease. Moreover, lymphocytes and inflammation factors significantly gradually increased. The level of HDL2b was negatively associated with WBC, lymphocytes and hs-CRP in multivariable linear regression analysis. In multinomial logistic regression analysis, high levels of HDL3 and low levels of HDL2b increased the probability of having an unfavourable metabolic unhealth/obesity status. We supposed that HDL2b particles may play anti-inflammation by negatively regulating lymphocytes activation. HDL2b may be a therapeutic target for future metabolic disease due to the anti-inflammatory effects.

中文翻译：

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抗炎 HDL 亚类在代谢不健康/肥胖中的潜在作用。


高密度脂蛋白（HDL）颗粒包含不同功能的异质亚类，通过与免疫反应细胞相互作用发挥抗炎作用。然而，HDL 亚类与代谢不健康/肥胖症中免疫反应细胞的关系尚未明确定义。本研究的目的是描绘代谢不健康/肥胖中 HDL 亚类与免疫细胞和炎症标志物的关系变化，以了解抗炎 HDL 亚类的作用。共有 316 名参与者按代谢健康状况进行分类。通过微流控芯片电泳检测HDL亚类。使用自动血液分析仪评估白细胞（WBC）计数和淋巴细胞。测量高敏 C 反应蛋白 (hs-CRP) 和白细胞介素 6 (IL-6) 的水平。在我们的研究中，随着代谢疾病的恶化，不仅HDL亚类的分布发生变化，HDL相关结构蛋白也发生变化。而且淋巴细胞和炎症因子逐渐明显增多。多变量线性回归分析显示HDL2b水平与WBC、淋巴细胞和hs-CRP呈负相关。在多项逻辑回归分析中，高水平的 HDL3 和低水平的 HDL2b 增加了不利的代谢不健康/肥胖状态的可能性。我们推测HDL2b颗粒可能通过负向调节淋巴细胞活化而发挥抗炎作用。由于其抗炎作用，HDL2b 可能成为未来代谢疾病的治疗靶点。