Objective: To study the expression and clinical value of PD-L1 gene in pancreatic cancer, and to predict the role of PD-L1 gene in the development of pancreatic cancer./nMethods: The pancreatic cancer datasets were downloaded from the Cancer Genome Atlas (TCGA) and the Oncomine to obtain the PD-L1 gene expression profile and clinical information. Bioinformatics methods were used to analyze the correlation between the expression level of PD-L1 gene in pancreatic cancer and clinicopathological indicators, as well as its influence on prognosis. GSEA and WGCNA analysis was performed to predict the possible pathways of PD-L1 gene regulation in pancreatic cancer. TIMER and MCP-counter were used for PD-L1 with immune infiltration. The genes interact with PD-L1 were also investigated by STING and immunoco-precipitation combined with mass spectrometry analysis (IP-MS)./nResults: In TCGA database, the overall survival of patients with high expression of PD-L1 gene was significantly lower than that of patients with low expression of PD-L1 gene (χ² = 12.52, P < 0.001). The samples with high expression of PD-L1 gene showed enrichment of 8 pathways including toll-like receptor signaling pathway and NOD receptor signaling pathway (P < 0.01, FDR < 0.05). Immune infiltration analysis suggested that PD-L1 were associated with monocytic lineage (r = 0.5). The proteins interacting with PD-L1 are mainly concentrated in RNA binding, ribosome, spliceosome and other biological processes or pathways./nConclusion: PD-L1 gene may play an important role in the development of pancreatic cancer and is expected to be a prognostic indicator of pancreatic cancer.

中文翻译：

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胰腺癌中 PD-L1 相关基因表达谱和肿瘤浸润淋巴细胞

目的：研究PD-L1基因在胰腺癌中的表达及临床价值，预测PD-L1基因在胰腺癌发生发展中的作用。/n方法：从癌症基因组图谱（TCGA）和 Oncomine 下载胰腺癌数据集，以获得 PD-L1 基因表达谱和临床信息。采用生物信息学方法分析胰腺癌中PD-L1基因表达水平与临床病理指标的相关性及其对预后的影响。进行 GSEA 和 WGCNA 分析以预测胰腺癌中 PD-L1 基因调控的可能途径。TIMER 和 MCP-counter 用于免疫浸润的 PD-L1。还通过 STING 和免疫共沉淀结合质谱分析 (IP-MS) 研究了与 PD-L1 相互作用的基因。/n结果：在TCGA数据库中，PD-L1基因高表达患者的总生存期明显低于PD-L1基因低表达患者（χ ² = 12.52，P < 0.001）。PD-L1基因高表达的样本富集了toll样受体信号通路和NOD受体信号通路8条通路（P < 0.01，FDR < 0.05）。免疫浸润分析表明 PD-L1 与单核细胞谱系相关 (r = 0.5)。用PD-L1的相互作用蛋白质主要集中在RNA结合，核糖体，剪接和其它生物过程或pathways./n结论： PD-L1基因可以在胰腺癌的发展中起着重要作用，并且预期是预后胰腺癌的指标。