Abstract

While current group I ribozymes use several distinct strategies to function under conditions of low Mg²⁺ concentration (≤ 3 mM), a deletion mutant of the Tetrahymena ribozyme (ΔP5 ribozyme) is virtually inactive with 3 mM Mg²⁺ due to removal of the large peripheral module, P5abc, supporting the active conformation of the core module. We investigated the molecular crowding effects of synthetic polyethylene glycols (PEGs) on the activity of the ΔP5 ribozyme. Among PEG molecules with different chain lengths, PEG600 improved the activity of the ΔP5 ribozyme most effectively in the presence of 3 mM Mg²⁺.

中文翻译：

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聚乙二醇分子簇链长对缺乏大外围模块的突变型四膜虫 I 型核酶的影响

摘要

虽然当前的 I 组核酶使用几种不同的策略在低 Mg ²⁺浓度（≤ 3 mM）的条件下发挥作用，但四膜虫核酶（ΔP5 核酶）的缺失突变体在3 mM Mg ^{2+ 中}由于去除了大型外围模块P5abc，支持核心模块的主动构象。我们研究了合成聚乙二醇 (PEG) 对 ΔP5 核酶活性的分子拥挤效应。在具有不同链长的PEG分子中，PEG600在3mM Mg ²⁺存在下最有效地提高ΔP5核酶的活性。