ABSTRACT

Background: Electrolytes, proteins, and other salivary molecules play an important role in tooth integrity and can serve as biomarkers associated with caries.

Objective: To determine the concentration of potential biomarkers in children without caries (CF) and children with caries (CA).

Methods: Unstimulated saliva was collected, and the biomarkers quantified in duplicate, using commercial Enzyme Linked Immunosorbent Assay (ELISA) kits to determine IgA, fibronectin, cathelicidin LL-37, and statherin levels, as well as colorimetric tests to detect formate and phosphate.

Results: Significantly higher concentrations of statherin was detected in the CF group (Median: 94,734.6; IQR: 92,934.6–95,113.7) compared to the CA2 group (90,875.0; IQR: 83,580.2–94,633.4) (p = 0.03). Slightly higher median IgA (48,250.0; IQR: 31,461.9–67,418.8) and LL-37 levels (56.1; IQR 43.6–116.2) and a lower concentration of formate were detected in the CF group (0.02; IQR 0.0034–0.15) compared to the group with caries (IgA: 37,776.42; IQR: 33,383.9–44,128.5; LL-37: 46.3; IQR: 40.1011–67.7; formate: 0.10; IQR: 0.01–0.18), but these differences were not statistically significant.

Conclusion: The fact that these compounds have been identified as good markers for caries among European adults highlights the difficulty of identifying universal biomarkers that are applicable to all ages or to different populations.

 抽象的

背景：电解质、蛋白质和其他唾液分子在牙齿完整性中发挥着重要作用，并且可以作为与龋齿相关的生物标志物。

目的：确定无龋儿童（CF）和有龋儿童（CA）中潜在生物标志物的浓度。

方法：收集未刺激的唾液，并使用商业酶联免疫吸附测定 (ELISA) 试剂盒测定 IgA、纤连蛋白、导管素 LL-37 和富酪蛋白水平，并使用比色测试检测甲酸盐和磷酸盐，对生物标志物进行重复定量。

结果：与 CA2 组（90,875.0；IQR：83,580.2–94,633.4）相比，CF 组检测到的富酪蛋白浓度显着更高（中位数：94,734.6；IQR：92,934.6–95,113.7）（p = 0.03）。与对照组相比，CF 组检测到 IgA 中位数（48,250.0；IQR：31,461.9–67,418.8）和 LL-37 水平（56.1；IQR 43.6–116.2）略高，甲酸浓度较低（0.02；IQR 0.0034–0.15）与龋齿相比（IgA：37,776.42；IQR：33,383.9–44,128.5；LL-37：46.3；IQR：40.1011–67.7；甲酸：0.10；IQR：0.01–0.18），但这些差异没有统计学意义。

结论：这些化合物已被确定为欧洲成年人龋齿的良好标志物，这一事实凸显了确定适用于所有年龄段或不同人群的通用生物标志物的难度。