Sarcocystosis is a significant meat borne coccidian disease with immense zoonotic potential. Sarcocystis fusiformis is the most prevalent Sarcocystis spp. affecting buffaloes across the globe. Most of the molecular characterization works on S. fusiformis are from Egypt and there is no record of such work from India. In the present study, 21 isolates of S. fusiformis from Northern India were characterized for 18S rRNA (MF595821-MF595841) and cox 1 (MF423105-MF423119 and MH899162-MH899167) genes. S. fusiformis was seen as a monophyletic sister group to S. cafferi on the phylogenetic tree comprising of different Sarcocystis spp. Both genes placed S. fusiformis close to those Sarcocystis spp. which have felids as definitive hosts in comparison to those with canids as definitive host. A total of 15 and 7 haplotypes were noticed for both the genes, respectively. The studied Indian isolates showed 99.1–100.0% and 99.2–100.0% nucleotide homologies within themselves for both the respective gene loci. Over all, cox 1 gene was found to be better in delineating the evolutionary phylogenetics in comparison to 18S rRNA gene. The findings are important from evolutionary point of view.

肉孢子虫病是一种重要的肉源性球虫病，具有巨大的人畜共患潜力。Sarcocystis fusiformis是最流行的Sarcocystis spp。影响全球的水牛。S. fusiformis 的大部分分子表征工作来自埃及，印度没有此类工作的记录。在本研究中，对来自印度北部的21 株梭形链球菌进行了 18S rRNA (MF595821-MF595841) 和 cox 1 (MF423105-MF423119 和 MH899162-MH899167) 基因的表征。S. fusiformis在由不同Sarcocystis spp组成的系统发育树上被视为S. cafferi的单系姐妹群。两个基因都被放置S. fusiformis接近那些Sarcocystis spp。与犬科动物作为终宿主的动物相比，猫科动物作为终宿主。对于这两个基因，分别注意到总共 15 个和 7 个单倍型。所研究的印度分离株在其各自的基因座中显示出 99.1-100.0% 和 99.2-100.0% 的核苷酸同源性。总的来说，与 18S rRNA 基因相比，cox 1 基因在描述进化系统发育方面表现更好。从进化的角度来看，这些发现很重要。