Genetic polymorphism in pathogen recognition receptors tends to influence infection, disease susceptibility, and progression. We analyzed the association of TLR4 and TLR9 gene polymorphisms with multiple hrHPV infections and HPV16 copy number in cervicitis and cervical cancer. A total of 440 cervical cancer, cervicitis, and healthy individuals were studied using PCR-based assays. Student t-test, chi-square test, Welch's t-test, and Fisher's Exact test were utilized to evaluate the association of HPV infection with polymorphisms. Haploview and FAMHAP were used to analyze haplotype association with HPV infection and viral load. Study results revealed HPV45 infection as the most common one in cervical cancer after HPV16, and one-fourth HPV positive cervical cancer patients possessed multiple HPV infections. Mean HPV16 copy number of 264.4 ± 58.7 and 2.1 ± 3.3 copies/cell was detected in cervical cancer and cervicitis, respectively. TLR4 rs10759931 was protective against multiple hrHPV infections. TLR4 haplotype ACAC was associated with an increased risk of multiple hrHPV infections. TLR9 SNPs rs187084, rs352140, and rs352139 were associated with decreased risk of high HPV16 copy number. Augmentation of efforts for the multivalent HPV vaccination in India is suggested. The analyzed polymorphisms were shown to modulate hrHPV co-infections and HPV16 viral load that warrants further analysis.

病原体识别受体的遗传多态性往往会影响感染、疾病易感性和进展。我们分析了TLR4和TLR9基因多态性与宫颈炎和宫颈癌中多种 hrHPV 感染和 HPV16 拷贝数的关系。使用基于 PCR 的检测方法研究了总共 440 名宫颈癌、宫颈炎和健康个体。学生 t 检验、卡方检验、Welch t检验-检验和Fisher精确检验用于评估HPV感染与多态性的关联。Haploview 和 FAMHAP 用于分析单倍型与 HPV 感染和病毒载量的关联。研究结果显示，HPV45感染是继HPV16之后最常见的一种宫颈癌，四分之一的HPV阳性宫颈癌患者有多种HPV感染。在宫颈癌和宫颈炎中分别检测到 264.4 ± 58.7 和 2.1 ± 3.3 拷贝/细胞的平均 HPV16 拷贝数。TLR4 rs10759931 对多种 hrHPV 感染具有保护作用。TLR4单倍型 ACAC 与多种 hrHPV 感染的风险增加有关。TLR9SNP rs187084、rs352140 和 rs352139 与高 HPV16 拷贝数风险降低相关。建议在印度加大多价 HPV 疫苗接种的力度。分析的多态性显示调节 hrHPV 共感染和 HPV16 病毒载量，值得进一步分析。