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A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-08-17 , DOI: 10.1111/jfbc.13871 Jian-Qiu Tu 1 , Hui-Ping Liu 1 , Ya-Hui Wen 1 , Pei Chen 1 , Zi-Tian Liu 1
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-08-17 , DOI: 10.1111/jfbc.13871 Jian-Qiu Tu 1 , Hui-Ping Liu 1 , Ya-Hui Wen 1 , Pei Chen 1 , Zi-Tian Liu 1
Affiliation
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A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study. We researched its structure and thermal stabilities, and further studied its antioxidant activities in vitro. The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography. Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01. Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal. The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃. In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals. It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc). Therefore, it was revealed that HEP might be used as a natural antioxidant component.
中文翻译：
猴头菇新型多糖的制备、结构特征、热稳定性和体外抗氧化活性
猴头菇的一种新型多糖组分 (HEP)在本研究中先后被分离和纯化。我们研究了其结构和热稳定性，并进一步研究了其体外抗氧化活性。结果表明，HEP是一种酸性杂多糖，高效凝胶渗透色谱的平均分子量约为19.7 kDa。离子色谱表明HEP主要由岩藻糖：半乳糖：葡萄糖：甘露糖：葡萄糖酸（Fuc：Gal：Glu：Man：GlcA）组成，摩尔比为1：2.87：0.09：0.12：0.01。此外，傅里叶变换红外光谱和核磁共振光谱进一步证明，HEP是一种含有α-构型的吡喃糖，主要由α-1-4-Fuc和α-1-6-Gal为主链组成，→3,6) -α-D-Man-(1→and→1,6)-Glc 分支，α-D-GlcpA-(1为T-末端。HEP的比旋光度为+55°；通过差示扫描量热法和HEP热重分析热稳定性测定表明，HEP的热解过程主要分为两个过程，其熔点为75.93℃。体外抗氧化实验表明，HEP具有一定的清除DPPH、羟基、超氧阴离子和ABTS自由基的能力。发现HEP具有很强的清除DPPH自由基的能力，最高清除率可达91.72%±0.17%，与维生素C(Vc)的清除能力基本相当。因此，揭示了 HEP 可能用作天然抗氧化剂成分。熔点75.93℃。体外抗氧化实验表明，HEP具有一定的清除DPPH、羟基、超氧阴离子和ABTS自由基的能力。发现HEP具有很强的清除DPPH自由基的能力，最高清除率可达91.72%±0.17%，与维生素C(Vc)的清除能力基本相当。因此，揭示了 HEP 可能用作天然抗氧化剂成分。熔点75.93℃。体外抗氧化实验表明，HEP具有一定的清除DPPH、羟基、超氧阴离子和ABTS自由基的能力。发现HEP具有很强的清除DPPH自由基的能力，最高清除率可达91.72%±0.17%，与维生素C(Vc)的清除能力基本相当。因此，揭示了 HEP 可能用作天然抗氧化剂成分。
更新日期：2021-09-06
中文翻译：
猴头菇新型多糖的制备、结构特征、热稳定性和体外抗氧化活性
猴头菇的一种新型多糖组分 (HEP)在本研究中先后被分离和纯化。我们研究了其结构和热稳定性，并进一步研究了其体外抗氧化活性。结果表明，HEP是一种酸性杂多糖，高效凝胶渗透色谱的平均分子量约为19.7 kDa。离子色谱表明HEP主要由岩藻糖：半乳糖：葡萄糖：甘露糖：葡萄糖酸（Fuc：Gal：Glu：Man：GlcA）组成，摩尔比为1：2.87：0.09：0.12：0.01。此外，傅里叶变换红外光谱和核磁共振光谱进一步证明，HEP是一种含有α-构型的吡喃糖，主要由α-1-4-Fuc和α-1-6-Gal为主链组成，→3,6) -α-D-Man-(1→and→1,6)-Glc 分支，α-D-GlcpA-(1为T-末端。HEP的比旋光度为+55°；通过差示扫描量热法和HEP热重分析热稳定性测定表明，HEP的热解过程主要分为两个过程，其熔点为75.93℃。体外抗氧化实验表明，HEP具有一定的清除DPPH、羟基、超氧阴离子和ABTS自由基的能力。发现HEP具有很强的清除DPPH自由基的能力，最高清除率可达91.72%±0.17%，与维生素C(Vc)的清除能力基本相当。因此，揭示了 HEP 可能用作天然抗氧化剂成分。熔点75.93℃。体外抗氧化实验表明，HEP具有一定的清除DPPH、羟基、超氧阴离子和ABTS自由基的能力。发现HEP具有很强的清除DPPH自由基的能力，最高清除率可达91.72%±0.17%，与维生素C(Vc)的清除能力基本相当。因此，揭示了 HEP 可能用作天然抗氧化剂成分。熔点75.93℃。体外抗氧化实验表明，HEP具有一定的清除DPPH、羟基、超氧阴离子和ABTS自由基的能力。发现HEP具有很强的清除DPPH自由基的能力，最高清除率可达91.72%±0.17%，与维生素C(Vc)的清除能力基本相当。因此，揭示了 HEP 可能用作天然抗氧化剂成分。
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