Microchimica Acta ( IF 5.3 ) Pub Date : 2021-08-16 , DOI: 10.1007/s00604-021-04929-4 Xue Sun 1, 2 , Minwen Jiang 1 , Ligang Chen 1 , Na Niu 1, 3
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A new type of ratiometric molecularly imprinted fluorescence probe (B-CQDs@Eu/MIPs) based on biomass carbon quantum dots (B-CQDs) and europium ions (Eu3+) has been prepared to recognize and detect tetracycline (TC). In the experiment, the fluorescent material B-CQDs were prepared using passion fruit peels through microwave-assisted method, which by the meantime achieves the reuse of biomass waste. TC can block the transition of some parts of electrons in the prepared B-CQDs from the excited state to the ground state, resulting in the weakening of its blue light (Ex = 394 nm, Em = 457 nm), while TC can be chelated by Eu3+ and emit red characteristic fluorescence (Ex = 394 nm, Em = 620 nm) due to the antenna effect. Thus, a ratiometric fluorescence response to TC is the result of the combined B-CQD and Eu3+ . Based on this, we established the ratiometric fluorescent molecularly imprinted (MIP) probe for the detection of TC. The prepared B-CQDs@Eu/MIPs is aimed at catching the fluorescence changes of target tetracycline (TC) sensitively with the special combination of the specific recognition cavities and TC. The linear fluorescence quenching range of TC in milk using the fluorescent probe was 25–2000 nM, and the detection limit was 7.9 nM. The recoveries of this method for TC were 94.2–103.7%, and the relative standard deviations (RSDs) were 1.5–5.3%. Owing to the predetermined nature of MIP technology and the special response of ratio fluorescence, the interference of common substances is eliminated completely, which greatly improved the selectivity of its practical applications.
Graphical abstract
中文翻译：
基于百香果皮碳点和铕选择性检测四环素的比例荧光MIPs探针的构建
制备了一种基于生物质碳量子点（B-CQDs）和铕离子（Eu 3+）的新型比例分子印迹荧光探针（B-CQDs@Eu/MIPs），用于识别和检测四环素（TC）。实验中，利用百香果皮通过微波辅助的方法制备了荧光材料B-CQDs，同时实现了生物质废弃物的再利用。TC可以阻断制备的B-CQDs中部分电子从激发态到基态的跃迁，导致其蓝光减弱（Ex = 394 nm，Em = 457 nm），而TC可以螯合由欧盟3+并由于天线效应发射红色特征荧光（Ex = 394 nm，Em = 620 nm）。因此，对 TC 的比率荧光响应是 B-CQD 和 Eu 3+组合的结果. 在此基础上，我们建立了用于检测TC的比率荧光分子印迹（MIP）探针。制备的B-CQDs@Eu/MIPs旨在通过特异性识别腔和TC的特殊组合，灵敏地捕捉目标四环素（TC）的荧光变化。使用荧光探针检测牛奶中TC的线性荧光猝灭范围为25-2000 nM，检测限为7.9 nM。该方法对 TC 的回收率为 94.2-103.7%，相对标准偏差 (RSD) 为 1.5-5.3%。由于MIP技术的预定性和比值荧光的特殊响应，完全消除了常见物质的干扰，大大提高了其实际应用的选择性。
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