Assembly of IgG-like asymmetric bispecific antibodies (bsAbs) requires heavy chain heterodimerization and cognate heavy-light chain pairings. Multiple strategies have been developed to solve these chain association issues. While these strategies greatly promote correct chain pairing, they normally cannot prevent low amount of chain mispaired byproducts from being generated. Besides, byproducts can also be generated as a result of discordant chain expression. The existence of various byproducts poses considerable challenges to downstream processing during the production of recombinant IgG-like bsAbs. In many cases, yield is greatly compromised for purity improvement. This mini review introduces eight IgG-like bsAb platforms, which share a common feature: they all contain built-in purification-facilitating elements in addition to chain pairing control designs. These platforms, by simultaneously providing solutions to the two issues associated with bsAb production (i.e., correct chain pairing and efficient purification), improve both efficiency and robustness of bsAb production.

IgG 样不对称双特异性抗体 (bsAbs) 的组装需要重链异二聚化和同源重轻链配对。已经开发了多种策略来解决这些链关联问题。虽然这些策略极大地促进了正确的链配对，但它们通常无法防止产生少量的链错误配对副产品。此外，由于链表达不一致，也会产生副产物。在重组 IgG 样 bsAb 的生产过程中，各种副产物的存在给下游加工带来了相当大的挑战。在许多情况下，产量会因纯度提高而大打折扣。这篇简短的评论介绍了八种 IgG 样 bsAb 平台，它们有一个共同的特点：除了链配对控制设计之外，它们都包含内置的促进净化的元素。这些平台通过同时为与 bsAb 生产相关的两个问题（即正确的链配对和有效纯化）提供解决方案，提高了 bsAb 生产的效率和稳健性。