Abstract

The study demonstrates the lab-scale production of biodiesel from soybean acid oil (SAO) in a 5 L lab-scale bioreactor using immobilized whole cells as a catalyst. The maximum lipase activity 2.89 ± 0.6 U/mL of immobilized whole cells was achieved at optimum conditions viz; pH (7.1), temperature (31 °C), peptone (1.2% w/v) and immobilized pieces (10) in 100 mL growth medium. The esterification approach was fit to Michaelis–Menten model, based on concentration-time profile with 75 gL⁻¹ Michaelis constant (K_M) and 78 gL⁻¹h⁻¹ maximum velocities (V_max) resulting in a maximum yield of up to 95.4% over 24 h of reaction. A 5% decrease in the catalytic potential of immobilized whole cells from 1^st to 5^th recycle of immobilized support. The fuel properties of resulted biodiesel were noted to meet the specifications of major international biodiesel standards such as ASTM D6751 to a significant extent. The study reveals the potential use of immobilized whole-cell catalyst and acid oil as an alternative, low-cost bearing and quality feedstock for the generation of biodiesel for a diverse variety of industrial/commercial use.

摘要

该研究展示了在 5 L 实验室规模的生物反应器中使用固定化全细胞作为催化剂，从大豆酸油 (SAO) 中实验室规模生产生物柴油。在最佳条件下，固定化全细胞的最大脂肪酶活性为 2.89 ± 0.6 U/mL；pH (7.1)、温度 (31 °C)、蛋白胨 (1.2% w/v) 和 100 mL 生长培养基中的固定片段 (10)。酯化方法符合 Michaelis-Menten 模型，基于浓度-时间曲线，75 gL ^-1 Michaelis 常数 ( K _M ) 和 78 gL ^-1 h ^-1最大速度 ( V _max) 在反应 24 小时内产生高达 95.4% 的最大产率。在固定化全细胞的催化电位A下降5％，从1^日至5^日的固定化支持再循环。注意到所得生物柴油的燃料特性在很大程度上满足主要国际生物柴油标准（例如ASTM D6751）的规格。该研究揭示了固定化全细胞催化剂和酸性油作为替代、低成本轴承和优质原料的潜在用途，用于生产用于各种工业/商业用途的生物柴油。