Protecting group chemistry for the cysteine thiol group has enabled a vast array of peptide and protein chemistry over the last several decades. Increasingly sophisticated strategies for the protection, and subsequent deprotection, of cysteine have been developed, facilitating synthesis of complex disulfide-rich peptides, semisynthesis of proteins, and peptide/protein labelling in vitro and in vivo. In this review, we analyse and discuss the 60+ individual protecting groups reported for cysteine, highlighting their applications in peptide synthesis and protein science.

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半胱氨酸保护基团：在肽和蛋白质科学中的应用

在过去的几十年里，半胱氨酸硫醇基团的保护基团化学使大量的肽和蛋白质化学成为可能。已经开发出越来越复杂的半胱氨酸保护和脱保护策略，促进了复杂的富含二硫键的肽的合成、蛋白质的半合成以及体外和体内的肽/蛋白质标记。在这篇综述中，我们分析和讨论了 60 多个针对半胱氨酸报道的独立保护基团，重点介绍了它们在肽合成和蛋白质科学中的应用。