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Prodigiosin Production and Photoprotective/Antigenotoxic Properties in Serratia marcescens Indigenous Strains from Eastern Cordillera of Colombia
Photochemistry and Photobiology ( IF 2.6 ) Pub Date : 2021-08-17 , DOI: 10.1111/php.13507 José D D Cediel Becerra 1 , Jhon Alexander Suescún Sepúlveda 1 , Jorge Luis Fuentes 1
Photochemistry and Photobiology ( IF 2.6 ) Pub Date : 2021-08-17 , DOI: 10.1111/php.13507 José D D Cediel Becerra 1 , Jhon Alexander Suescún Sepúlveda 1 , Jorge Luis Fuentes 1
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Serratia marcescens is a bacterial species that produces an antibacterial pigment (Prodigiosin) showing a wide adaptive response to environmental stresses. The study aimed to investigate Prodigiosin production in S. marcescens wild-type strains, as well as its relation to photoprotection and antigenotoxicity against UVB. Prodigiosin yield was spectrophotometrically assayed in extracts of bacterial strains grown in different culture media. In vitro photoprotection efficacy was evaluated using the in vitro indices sun protection factor (SPFin vitro) and critical wavelength (λc). The percentage of UVB antigenotoxicity estimates (%GI) in the SOS Chromotest was also evaluated. Correlation analysis was used to examine the relationship between Prodigiosin yield, SPFin vitro, %GI estimates and environmental traits (altitude, temperature, rainfall and solar irradiance). Prodigiosin yield in S. marcescens strains varied depending on culture media used for its growth, and it was correlated with environmental variables such as temperature and solar irradiance. SPFin vitro estimates were well correlated with Prodigiosin concentration and %GI values in the bacterial strains being studied. UVB photoprotective efficacy of the extracts obtained from S. marcescens strains depends on the strain’s Prodigiosin yield and its antigenotoxic potential. The extracts with Prodigiosin yield higher than ~17 μg mL−1 could be used as sources of sunscreen ingredients.
中文翻译：
哥伦比亚东部科迪勒拉地区粘质沙雷氏菌本土菌株的灵菌红素生产和光保护/抗毒性
Serratia marcescens是一种产生抗菌色素（Prodigiosin）的细菌，对环境压力表现出广泛的适应性反应。该研究旨在调查粘质沙门氏菌野生型菌株中灵菌红素的产生，以及它与对 UVB 的光保护和抗原毒性的关系。在不同培养基中生长的细菌菌株的提取物中用分光光度法测定灵菌红素的产量。使用体外指数防晒系数（SPF体外）和临界波长（λc）。还评估了 SOS Chromotest 中 UVB 抗原毒性估计值 (%GI) 的百分比。相关性分析用于检查灵芝红素产量、体外SPF 、%GI 估计值和环境特征（海拔、温度、降雨量和太阳辐照度）之间的关系。S. marcescens菌株中的灵珠红素产量取决于用于其生长的培养基，并且与温度和太阳辐照度等环境变量相关。SPF体外估计值与正在研究的细菌菌株中的灵珠红素浓度和 %GI 值密切相关。从粘质沙门氏菌中获得的提取物的 UVB 光保护功效菌株取决于菌株的灵菌红素产量及其抗原毒性潜力。灵菌红素产量高于~17 μg mL -1的提取物可用作防晒成分的来源。
更新日期：2021-08-17
中文翻译：
哥伦比亚东部科迪勒拉地区粘质沙雷氏菌本土菌株的灵菌红素生产和光保护/抗毒性
Serratia marcescens是一种产生抗菌色素（Prodigiosin）的细菌，对环境压力表现出广泛的适应性反应。该研究旨在调查粘质沙门氏菌野生型菌株中灵菌红素的产生，以及它与对 UVB 的光保护和抗原毒性的关系。在不同培养基中生长的细菌菌株的提取物中用分光光度法测定灵菌红素的产量。使用体外指数防晒系数（SPF体外）和临界波长（λc）。还评估了 SOS Chromotest 中 UVB 抗原毒性估计值 (%GI) 的百分比。相关性分析用于检查灵芝红素产量、体外SPF 、%GI 估计值和环境特征（海拔、温度、降雨量和太阳辐照度）之间的关系。S. marcescens菌株中的灵珠红素产量取决于用于其生长的培养基，并且与温度和太阳辐照度等环境变量相关。SPF体外估计值与正在研究的细菌菌株中的灵珠红素浓度和 %GI 值密切相关。从粘质沙门氏菌中获得的提取物的 UVB 光保护功效菌株取决于菌株的灵菌红素产量及其抗原毒性潜力。灵菌红素产量高于~17 μg mL -1的提取物可用作防晒成分的来源。
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