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Aspergillus Section Flavi from Four Agricultural Products and Association of Mycotoxin and Sclerotia Production with Isolation Source
Current Microbiology ( IF 2.3 ) Pub Date : 2021-08-16 , DOI: 10.1007/s00284-021-02620-8
Azadeh Habibi 1 , Daryoush Afzali 2
Affiliation  

Many agricultural products are susceptible to contamination by aflatoxin-producing species from Aspergillus section Flavi. The objectives of this study were to determine the occurrence of Aspergillus section Flavi in four agricultural products, such as pistachio, walnut, hazelnut, and dried fruits, collected from market and retail shops in various areas of Kerman County and obtain information on the relationships between isolation source and ability to produce sclerotia and potential for aflatoxin production. Aspergillus species were identified based on morphological characteristics as well as subsequent sequencing of the parts of the β-tubulin and calmodulin genes. From 207 isolated strains, the following species were identified: A. flavus, A. tamarii A. nomius, A. parasiticus, A. arachidicola, A. caelatus, A. pseudotamarii, and A. leporis. To the best of our knowledge, this is the first report of A. pseudotamarii and A. arachidicola with the potential to produce aflatoxins from dried apricots and hazelnuts, respectively. Sclerotial type was significantly different between isolates from different isolation sources. From 192 tested isolates, 38% were aflatoxin producer from which 5% were scored as strong aflatoxin producers and 33% as average aflatoxin producers. A significant difference in the population of aflatoxin-producing strains across the isolation sources was observed which may reflect host adaptation and thereby different vulnerabilities to aflatoxin-producing species among the examined products.



中文翻译:

四种农产品黄曲霉切片与真菌毒素和菌核产生与分离来源的关联

许多农产品容易受到来自Aspergillus section Flavi的产黄曲霉毒素物种的污染。这项研究的目的是确定的发生曲霉部分黄热病四个农产品,如开心果,核桃,榛子,水果和干果,从克尔曼县的各个领域的市场和零售商店收集和获得之间的关系的信息分离来源和产生菌核的能力以及产生黄曲霉毒素的潜力。曲霉菌根据形态特征以及随后对 β-微管蛋白和钙调蛋白基因部分的测序,鉴定了不同的物种。从207个分离的菌株,以下物种鉴定:黄曲霉,溜曲霉A. nomius寄生曲霉A.花生A. caelatusA. pseudotamarii,A. leporis。据我们所知,这是A. pseudotamariiA. arachidicola的第一份报告分别从杏干和榛子中产生黄曲霉毒素的潜力。不同分离来源的分离株之间的菌核类型有显着差异。从 192 个测试分离株中,38% 是黄曲霉毒素生产者,其中 5% 被评为强黄曲霉毒素生产者,33% 是平均黄曲霉毒素生产者。观察到不同分离源的产黄曲霉毒素菌株数量存在显着差异,这可能反映了宿主适应性,从而反映了受检产品对产黄曲霉毒素物种的不同脆弱性。

更新日期:2021-09-13
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