The present study aimed to investigate the influence of circular RNA nuclear receptor‑interacting protein 1 (circNRIP1) on the chemotherapeutic effect of 5‑fluorouracil (5‑FU) in colorectal cancer (CRC) and reveal its potential molecular mechanisms. The effects of circNRIP1 on cell proliferation, migration and invasion, and apoptosis were evaluated using Cell Counting Kit‑8, Transwell and flow cytometric assays, respectively. A dual‑luciferase reporter assay was performed to verify the potential interaction between circNRIP1 and microRNA (miR)‑532‑3p. The results of the present study indicated that circNRIP1 was upregulated in CRC and its increased expression was associated with CRC progression. Furthermore, overexpression of circNRIP1 promoted CRC cell proliferation, invasion and migration, while it inhibited apoptosis. Knockdown of circNRIP1 significantly enhanced the 5‑FU‑induced inhibition of the viability of HCT116 and SW480 cells. Bioinformatics analysis predicted that miR‑532‑3p was a direct target of circNRIP1, which was further confirmed by a dual‑luciferase reporter assay. miR‑532‑3p silencing reversed the effects of circNRIP1 knockdown on the sensitivity of 5‑FU in the chemotherapy of CRC. The results suggested that circNRIP1 and miR‑532‑3p may be utilized to improve the diagnosis of CRC and serve as diagnostic markers. In conclusion, overexpression of circNRIP1 promoted the progression of CRC, while circNRIP1 silencing sensitized CRC cells to 5‑FU via sponging miR‑532‑3p.

中文翻译：

---

通过海绵 miR-532-3p 敲除 circNRIP1 使结直肠癌对 5-FU 敏感。

本研究旨在探讨环状 RNA 核受体相互作用蛋白 1 (circNRIP1) 对 5-氟尿嘧啶 (5-FU) 对结直肠癌 (CRC) 化疗效果的影响，并揭示其潜在的分子机制。分别使用 Cell Counting Kit-8、Transwell 和流式细胞术检测 circNRIP1 对细胞增殖、迁移和侵袭以及细胞凋亡的影响。进行了双荧光素酶报告基因检测以验证 circNRIP1 和 microRNA (miR)-532-3p 之间的潜在相互作用。本研究的结果表明 circNRIP1 在 CRC 中上调，其表达增加与 CRC 进展相关。此外，circNRIP1的过表达促进CRC细胞增殖、侵袭和迁移，同时抑制细胞凋亡。circNRIP1 的敲低显着增强了 5-FU 诱导的 HCT116 和 SW480 细胞活力的抑制。生物信息学分析预测 miR-532-3p 是 circNRIP1 的直接靶标，这一点得到了双荧光素酶报告基因检测的进一步证实。miR-532-3p 沉默逆转了 circNRIP1 敲低对 CRC 化疗中 5-FU 敏感性的影响。结果表明circNRIP1和miR-532-3p可用于改善CRC的诊断并作为诊断标志物。总之，circNRIP1的过表达促进了CRC的进展，而circNRIP1沉默通过海绵miR-532-3p使CRC细胞对5-FU敏感。双荧光素酶报告基因检测进一步证实了这一点。miR-532-3p 沉默逆转了 circNRIP1 敲低对 CRC 化疗中 5-FU 敏感性的影响。结果表明circNRIP1和miR-532-3p可用于改善CRC的诊断并作为诊断标志物。总之，circNRIP1的过表达促进了CRC的进展，而circNRIP1沉默通过海绵miR-532-3p使CRC细胞对5-FU敏感。双荧光素酶报告基因检测进一步证实了这一点。miR-532-3p 沉默逆转了 circNRIP1 敲低对 CRC 化疗中 5-FU 敏感性的影响。结果表明circNRIP1和miR-532-3p可用于改善CRC的诊断并作为诊断标志物。总之，circNRIP1的过表达促进了CRC的进展，而circNRIP1沉默通过海绵miR-532-3p使CRC细胞对5-FU敏感。