This article was designed to explore the effects and mechanisms of miR-185-5p on the replication of hepatitis C virus (HCV). Quantitative reverse transcription PCR (qRT-PCR) was performed for detecting the abundance of miR-185-5p and HCV RNA in HCV-infected primary hepatocytes and Huh7.5 cells. Dual-luciferase reporter gene assay was used for exploring the interaction between miR-185-5p and GALNT8. Western blot analyzed protein expression of GALNT8, NS3, and NS5A. miR-185-5p was remarkably downregulated in HCV-infected primary hepatocytes and Huh7.5 cells. miR-185-5p upregulation inhibited HCV RNA expression, while its inhibition promoted HCV replication. miR-185-5p induced accumulation of NS3 and NS5A in the cells. Dual-luciferase reporter gene assay verified the targeted relationship between miR-185-5p and GALNT8. In addition, the effects of overexpressing or knocking down miR-185-5p on HCV replication could be correspondingly eliminated by the overexpression or knockdown of GALNT8. miR-185-5p may target GALNT8 in JFH1-infected Huh7.5 cells and then inhibit HCV replication. miR-185-5p may be a potential target for treating HCV.

中文翻译：

---

miR-185-5p对丙型肝炎病毒复制的影响

本文旨在探讨miR-185-5p对丙型肝炎病毒（HCV）复制的影响和机制。进行定量逆转录 PCR (qRT-PCR) 以检测 HCV 感染的原代肝细胞和 Huh7.5 细胞中 miR-185-5p 和 HCV RNA 的丰度。双荧光素酶报告基因检测用于探索 miR-185-5p 和 GALNT8 之间的相互作用。蛋白质印迹分析了 GALNT8、NS3 和 NS5A 的蛋白质表达。miR-185-5p 在 HCV 感染的原代肝细胞和 Huh7.5 细胞中显着下调。miR-185-5p 上调抑制 HCV RNA 表达，而其抑制促进 HCV 复制。miR-185-5p 诱导细胞中 NS3 和 NS5A 的积累。双荧光素酶报告基因检测验证了 miR-185-5p 和 GALNT8 之间的靶向关系。此外，过表达或敲低 miR-185-5p 对 HCV 复制的影响可以通过 GALNT8 的过表达或敲低相应地消除。miR-185-5p 可能靶向 JFH1 感染的 Huh7.5 细胞中的 GALNT8，然后抑制 HCV 复制。miR-185-5p 可能是治疗 HCV 的潜在靶点。