ABSTRACT

Background

Glioma is the most common intrinsic tumor in central nervous system and is characterized by their diffuse infiltration of the brain tissue. Insulin-like Growth Factor Binding Protein-6 (IGFBP6) was associated with the insulin-like growth factor binding and insulin-like growth factor II binding processes in many cancers. Herein, we aimed to investigate the biological functions and clinical features of IGFBP6 in gliomas.

Methods

Totally, we collected 325 RNA sequencing data from CGGA dataset as training cohort, and 969 RNA sequencing data from TCGA dataset as validation cohort. The clinical and molecular characteristics analysis and gene ontology analysis of IGFBP6 were performed. All analyses and graphs were produced based on R language.

Results

We found that IGFBP6 expression was significantly upregulated in GBM patients and downregulated in IDH mutant patients. Receiver Operating Characteristic (ROC) analysis revealed that IGFBP6 could be used as a biomarker to predict TCGA mesenchymal subtype. GO analysis revealed that IGFBP6 was correlated with immunological functions and inflammation activities. Meanwhile, higher expression of IGFBP6 suggested significant relationship with worse prognosis in glioma patients.

Conclusions

Our findings improved the understanding of IGFBP6 in glioma, and IGFBP6 might be a potential therapeutic target for glioma patients in future clinical trials.

中文翻译：

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IGFBP6在胶质瘤中的临床特征及预后价值

摘要

背景

神经胶质瘤是中枢神经系统中最常见的内在肿瘤，其特点是弥漫性浸润脑组织。胰岛素样生长因子结合蛋白 6 (IGFBP6) 与许多癌症中的胰岛素样生长因子结合和胰岛素样生长因子 II 结合过程有关。在此，我们旨在研究 IGFBP6 在胶质瘤中的生物学功能和临床特征。

方法

我们总共从 CGGA 数据集中收集了 325 个 RNA 测序数据作为训练队列，从 TCGA 数据集中收集了 969 个 RNA 测序数据作为验证队列。进行了IGFBP6的临床和分子特征分析和基因本体分析。所有分析和图表均基于 R 语言生成。

结果

我们发现 IGFBP6 表达在 GBM 患者中显着上调，在 IDH 突变患者中下调。接受者操作特征 (ROC) 分析显示 IGFBP6 可用作预测 TCGA 间充质亚型的生物标志物。GO分析显示IGFBP6与免疫功能和炎症活动相关。同时，IGFBP6 的高表达表明与胶质瘤患者预后较差的显着相关。

结论

我们的研究结果提高了对胶质瘤中 IGFBP6 的认识，IGFBP6 可能成为未来临床试验中胶质瘤患者的潜在治疗靶点。