A variety of glycosides with interesting biological properties suitable for application in cosmetic and pharmaceutical industry can be found in nature. However, the demand exceeds the quantity and purity which can be obtained naturally and therefore enzymatic glycosylation has been of great interest in recent decades. In this study, crude Kluyveromyces lactis enzyme preparation Lactozym 3000 L was examined for the production of two phenolic glycosides – tyrosol β-fructoside and tyrosol β-galactoside. Its performance was compared to other commercial fungal and yeast enzyme preparations. It was found that the key enzyme, β-galactosidase is unsuitable for tyrosol β-galactoside production. However, one of the Lactozym 3000 L components, β-fructofuranosidase, performed very well in the tyrosol β-fructoside production from sucrose and tyrosol. Lactozym 3000 L was effectively immobilized onto activated carrier Lifetech ECR8309 via covalent binding of the enzyme, which is a promising method to reduce very high enzyme consumption in its free form. Potential of deep-eutectic solvents to enhance transfructosylation selectivity has been studied proving their interesting stabilization effect on Lactozym 3000 L β-fructofuranosidase.

自然界中可以找到多种具有有趣生物学特性的糖苷，适用于化妆品和制药行业。然而，需求超过了可以自然获得的数量和纯度，因此近几十年来酶促糖基化引起了人们的极大兴趣。在本研究中，研究了乳酸克鲁维酵母粗制酶制剂 Lactozym 3000 L 的两种酚糖苷——酪醇β-果糖苷和酪醇β-半乳糖苷的生产。将其性能与其他商业真菌和酵母酶制剂进行比较。发现关键酶β-半乳糖苷酶不适用于酪醇β-半乳糖苷生产。然而，Lactozym 3000 L个分量，一个β呋喃果糖苷酶，在酪醇表现非常出色β从蔗糖和酪醇-fructoside生产。Lactozym 3000 L 通过酶的共价结合有效地固定在活化的载体 Lifetech ECR8309 上，这是一种很有前途的方法，可以减少游离形式的酶消耗量。已经研究了深共熔溶剂增强转果糖基化选择性的潜力，证明了它们对 Lactozym 3000 L β-呋喃果糖苷酶的有趣稳定作用。