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Label-free single-cell isolation enabled by microfluidic impact printing and real-time cellular recognition
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-08-09 , DOI: 10.1039/d1lc00326g Yiming Wang 1, 2 , Xiaojie Wang 1, 2 , Tingrui Pan 1, 3 , Baoqing Li 1, 2 , Jiaru Chu 1, 2
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-08-09 , DOI: 10.1039/d1lc00326g Yiming Wang 1, 2 , Xiaojie Wang 1, 2 , Tingrui Pan 1, 3 , Baoqing Li 1, 2 , Jiaru Chu 1, 2
Affiliation
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Analysis of cellular components at the single-cell level is important to reveal cellular heterogeneity. However, current technologies to isolate individual cells are either label-based or have low performance. Here, we present a novel technique by integrating real-time cellular recognition and microfluidic impact printing (MIP) to isolate single cells with high efficiency and high throughput in a label-free manner. Specifically, morphological characteristics of polystyrene beads and cells, computed by an efficient image processing algorithm, are utilized as selection criteria to identify target objects. Subsequently, each detected single-cell object in the suspension is ejected from the microfluidic channel by impact force. It has been demonstrated that the single-cell isolating system has the ability to encapsulate polystyrene beads in droplets with an efficiency of 95%, while for HeLa cells, this has been experimentally measured as 90.3%. Single-cell droplet arrays are generated at a throughput of 2 Hz and 96.6% of the cells remain alive after isolation. This technology has significant potential in various emerging applications, including single-cell omics, tissue engineering, and cell-line development.
中文翻译：
通过微流体冲击打印和实时细胞识别实现无标记单细胞分离
在单细胞水平上分析细胞成分对于揭示细胞异质性很重要。然而，目前分离单个细胞的技术要么基于标签，要么性能低下。在这里，我们提出了一种新技术，通过集成实时细胞识别和微流体冲击打印 (MIP)，以无标记的方式以高效率和高通量分离单个细胞。具体来说，聚苯乙烯珠子和细胞的形态特征，通过有效的图像处理算法计算，被用作选择标准来识别目标对象。随后，悬浮液中每个检测到的单细胞物体通过冲击力从微流体通道中弹出。已经证明，单细胞隔离系统能够以 95% 的效率将聚苯乙烯珠封装在液滴中，而对于 HeLa 细胞，实验测量为 90.3%。单细胞液滴阵列以 2 Hz 的吞吐量生成，分离后 96.6% 的细胞保持存活。该技术在各种新兴应用中具有巨大潜力，包括单细胞组学、组织工程和细胞系开发。
更新日期：2021-08-16
中文翻译：
通过微流体冲击打印和实时细胞识别实现无标记单细胞分离
在单细胞水平上分析细胞成分对于揭示细胞异质性很重要。然而，目前分离单个细胞的技术要么基于标签，要么性能低下。在这里，我们提出了一种新技术，通过集成实时细胞识别和微流体冲击打印 (MIP)，以无标记的方式以高效率和高通量分离单个细胞。具体来说，聚苯乙烯珠子和细胞的形态特征，通过有效的图像处理算法计算，被用作选择标准来识别目标对象。随后，悬浮液中每个检测到的单细胞物体通过冲击力从微流体通道中弹出。已经证明，单细胞隔离系统能够以 95% 的效率将聚苯乙烯珠封装在液滴中，而对于 HeLa 细胞，实验测量为 90.3%。单细胞液滴阵列以 2 Hz 的吞吐量生成，分离后 96.6% 的细胞保持存活。该技术在各种新兴应用中具有巨大潜力，包括单细胞组学、组织工程和细胞系开发。
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