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A feasible self-assembled near-infrared fluorescence sensor for acid phosphatase detection and cell imaging
Analyst ( IF 3.6 ) Pub Date : 2021-08-03 , DOI: 10.1039/d1an01218e
Shuangqin Li 1 , Yaya Wang 1 , Shuai Mu 1 , Jinlong Zhang 1 , Xiaoyan Liu 1 , Syed Faheem Askari Rizvi 1 , Haixia Zhang 1 , Nana Ding 2 , Lan Wu 2
Affiliation  

The single signal amplification strategy is significant for detecting various disease biomarkers but is restricted by its limited accuracy. The multi-signal and multi-mode methods have overcome this deficiency. Acid phosphatase (ACP) is an important intracellular enzyme but one-step cell imaging material-based probes are scarce for ACP. Herein, we designed a one-step self-assembled polymer probe using neutral red (NR), modified-(pyridoxal-5′-phosphate (PLP)) and Eu3+. The polymer exhibited non-emission and excellent stability. Upon the catalytic hydrolysis reaction of ACP, the polymer exhibited two strong fluorescence signals at 373 nm and 613 nm and an appreciable decline of absorbance at 395 nm. The probe has excellent selectivity and higher sensitivity with a limit of detection as low as 0.02 mU mL−1. It possesses favorable biocompatibility and has been successfully used to detect and image intracellular ACP in several living cells.

中文翻译:

一种可行的自组装近红外荧光传感器用于酸性磷酸酶检测和细胞成像

单信号放大策略对于检测各种疾病生物标志物具有重要意义,但其准确性有限。多信号和多模式方法克服了这一不足。酸性磷酸酶 (ACP) 是一种重要的细胞内酶,但 ACP 缺乏基于一步细胞成像材料的探针。在此,我们设计了一种使用中性红 (NR)、改性-(pyridoxal-5'-磷酸盐 (PLP)) 和 Eu 3+的一步自组装聚合物探针。该聚合物表现出不发射和优异的稳定性。在 ACP 催化水解反应后,聚合物在 373 nm 和 613 nm 处显示出两个强烈的荧光信号,并且在 395 nm 处的吸光度明显下降。该探针具有出色的选择性和更高的灵敏度,检测限低至 0.02 mU mL-1。它具有良好的生物相容性,并已成功用于检测和成像多种活细胞中的细胞内 ACP。
更新日期:2021-08-16
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