A novel pine wood nematode effector, BxSCD1, suppresses plant immunity and interacts with an ethylene-forming enzyme in pine,Molecular Plant Pathology

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A novel pine wood nematode effector, BxSCD1, suppresses plant immunity and interacts with an ethylene-forming enzyme in pine
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-08-16 , DOI: 10.1111/mpp.13121
Tong-Yue Wen _{1,

2} , Xiao-Qin Wu _{1,

2} , Long-Jiao Hu _{1,

2} , Yi-Jun Qiu _{1,

2} , Lin Rui _{1,

2} , Yan Zhang _{1,

2} , Xiao-Lei Ding _{1,

2} , Jian-Ren Ye _{1,

2}

Affiliation

The plant-parasitic nematode Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), causes enormous economic loss every year. Currently, little is known about the pathogenic mechanisms of PWD. Several effectors have been identified in B. xylophilus, but their functions and host targets have yet to be elucidated. Here, we demonstrated that BxSCD1 suppresses cell death and inhibits B. xylophilus PAMP BxCDP1-triggered immunity in Nicotiana benthamiana and Pinus thunbergii. BxSCD1 was transcriptionally upregulated in the early stage of B. xylophilus infection. In situ hybridization experiments showed that BxSCD1 was specifically expressed in the dorsal glands and intestine. Cysteine residues are essential for the function of BxSCD1. Transient expression of BxSCD1 in N. benthamiana revealed that it was primarily targeted to the cytoplasm and nucleus. The morbidity was significantly reduced in P. thunbergii infected with B. xylophilus when BxSCD1 was silenced. We identified 1-aminocyclopropane-1-carboxylate oxidase 1, the actual ethylene-forming enzyme, as a host target of BxSCD1 by yeast two-hybrid and coimmunoprecipitation. Overall, this study illustrated that BxSCD1 played a critical role in the B. xylophilus–plant interaction.

中文翻译：

一种新型松木线虫效应器 BxSCD1 抑制植物免疫并与松树中的乙烯形成酶相互作用

植物寄生线虫松材线虫( Bursaphelenchus xylophilus )是松材枯萎病(PWD)的病原体，每年都会造成巨大的经济损失。目前，人们对 PWD 的发病机制知之甚少。在B中已经确定了几个效应器。 松材线虫，但它们的功能和宿主目标尚未阐明。在这里，我们证明了 BxSCD1在本氏烟草和松树中抑制细胞死亡并抑制松材线虫PAMP BxCDP1 触发的免疫。BxSCD1在松材线虫感染的早期被转录上调。原位杂交实验表明BxSCD1在背腺和肠道中特异性表达。半胱氨酸残基对 BxSCD1 的功能至关重要。BxSCD1 在本氏烟草中的瞬时表达表明它主要针对细胞质和细胞核。感染B的P. thunbergii的发病率显着降低。 BxSCD1沉默时的松材线虫。我们通过酵母双杂交和免疫共沉淀鉴定了 1-氨基环丙烷-1-羧酸氧化酶 1（实际的乙烯形成酶）作为 BxSCD1 的宿主靶标。总体而言，这项研究表明 BxSCD1 在松材线虫与植物的相互作用中发挥了关键作用。

更新日期：2021-10-15

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