Reduction of antibiotic-induced biofilm accumulation of Pseudomonas aeruginosa by quaternized phytoglycogen,Letters in Applied Microbiology

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Reduction of antibiotic-induced biofilm accumulation of Pseudomonas aeruginosa by quaternized phytoglycogen
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-08-16 , DOI: 10.1111/lam.13554
S R Schooling ₁ , K Klinger ₂ , A Korenevski ₂ , S Glasauer ₁

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Biofilms are oft cited as a factor in the unwanted persistence and recalcitrance of microbial life and a strong research initiative exists to identify, understand, and target vulnerabilities. Phytoglycogen is a biodegradable nanoparticulate biomaterial that is purified from crop plants. Importantly, the highly branched glucan structure provides a scaffold on which to construct novel polymers. Functionalized phytoglycogen (FP) was synthesized using green chemistry principles. Screening of several molecules identified a form of quaternized phytoglycogen which reduced biofilm formation and accretion by Pseudomonas aeruginosa. Exposing P. aeruginosa to modified phytoglycogen and antibiotic in combination not only substantively reduced biofilms, but also prevented increased biofilm formation, a biological response to suboptimal antibiotic concentrations. Treatment of pregrown biofilms with sub-minimum inhibitory concentration antibiotic alone also led to increased proliferation, whereas FP-antibiotic combinations prevented or reduced the extent of this. Swimming, swarming and twitching motility, all critical for biofilm development, were negatively affected by FP. This work supports phytoglycogen as a promising foundational molecule for novel polymers, including those with anti-biofilm function. Critically, in addition to published reports on how suboptimal antibiotic concentrations promote biofilm formation, we demonstrated a similar effect upon pre-existing biofilms, indicating a further route for the failure of antibiotic therapies.

中文翻译：

季铵化植物糖原减少抗生素诱导的铜绿假单胞菌生物膜积累

生物膜经常被认为是微生物生命不希望的持久性和顽固性的一个因素，并且存在一个强大的研究计划来识别、理解和瞄准漏洞。植物糖原是一种可生物降解的纳米颗粒生物材料，从农作物中提纯。重要的是，高度支化的葡聚糖结构为构建新型聚合物提供了支架。功能化植物糖原 (FP) 是使用绿色化学原理合成的。对几种分子的筛选确定了一种季铵化植物糖原的形式，它减少了铜绿假单胞菌的生物膜形成和增加。暴露铜绿假单胞菌将修饰的植物糖原和抗生素结合使用不仅可以显着减少生物膜，而且还可以防止生物膜形成增加，这是对次优抗生素浓度的生物反应。单独用亚最低抑制浓度抗生素处理预生长的生物膜也导致增殖增加，而 FP-抗生素组合阻止或减少了这种程度。游泳、蜂群和抽搐的运动性，对生物膜的发展都至关重要，都受到 FP 的负面影响。这项工作支持植物糖原作为新型聚合物的有前途的基础分子，包括具有抗生物膜功能的聚合物。至关重要的是，除了已发表的关于次优抗生素浓度如何促进生物膜形成的报告外，我们还证明了对预先存在的生物膜的类似影响，

更新日期：2021-08-16

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