Cytokinins (CTKs) enhance plant tolerance to heavy metals, but the associated mechanisms are not yet fully understood. Here, we investigated the effects of kinetin (KT, a type of CTKs) on arsenic (As) speciation and antioxidative enzymes in plant fronds, by comparing Pteris cretica var. nervosa (As hyperaccumulator) and Pteris ensiformis (non-hyperaccumulator). A hydroponic test was carried out and the two plant species were treated with 2 mg L⁻¹ arsenite [As(III)], arsenate [As(V)] or dimethylarsinate (DMA), and then 0–1.0 mg L⁻¹ KT was exogenously applied for 14 days. The maximum As uptake of the two experimental plants was 0.5 mg L⁻¹ KT. Fresh plant biomass was also significantly increased, except in P. cretica var. nervosa under DMA stress. The frond superoxide dismutase (SOD) and catalase (CAT) activity in the two plant species was significantly increased by 0.5 mg L⁻¹ KT. Additionally, a significant decline in frond peroxidase (POD) activity was observed in P. ensiformis at 1.0 mg L⁻¹ KT under As(III) stress. However, POD activity was significantly increased in P. cretica var. nervosa, at 0.1 mg L⁻¹ KT, under As(III) or DMA stress, and at 1.0 mg L⁻¹ KT, under As(V) stress. Therefore, KT promoted As transport and increased frond POD activity in P. cretica var. nervosa, which contributed to its As tolerance under stress from different As species.

细胞分裂素 (CTKs) 增强植物对重金属的耐受性，但相关机制尚未完全了解。在这里，我们通过比较Pteris cretica var.研究了激动素（KT，一种 CTK）对植物叶片中砷 (As) 形态和抗氧化酶的影响。nervosa (As hyperaccumulator) 和Pteris ensiformis (non-hyperaccumulator)。进行了水培试验，用 2 mg L ^-1亚砷酸盐 [As(III)]、砷酸盐 [As(V)] 或二甲基砷酸盐 (DMA)处理这两种植物，然后加入 0–1.0 mg L ^-1 KT外用14天。两个实验植物的最大As吸收量为0.5 mg L ^-1KT。新鲜植物生物量也显着增加，除了P. cretica var。DMA 压力下的神经性。两种植物的叶超氧化物歧化酶(SOD) 和过氧化氢酶(CAT) 活性显着增加0.5 mg L ^-1 KT。此外，在 As(III) 胁迫下，在 1.0 mg L ^-1 KT 的P. ensiformis中观察到叶过氧化物酶 (POD) 活性显着下降。然而，P. cretica var. 中的POD 活性显着增加。神经性，在 0.1 mg L ^-1 KT，在 As(III) 或 DMA 压力下，在 1.0 mg L ^-1KT，在 As(V) 应力下。因此，KT 促进了P. cretica var. 中的As 运输并增加了叶状 POD 活性。nervosa，这有助于其在来自不同 As 物种的压力下的 As 耐受性。