Although doxorubicin (DOX) is used in many cancer treatments, it causes neurotoxicity. In this study, the effect of thymoquinone (THQ), a powerful antioxidant, on DOX-induced neurotoxicity was evaluated. In total, 40 rats were used and 5 groups were formed. Group I: control group (n = 8); Group II: olive oil group (n = 8); Group III: the THQ group (n = 8); THQ 10 mg/kg per day was given intraperitoneally (i.p.) throughout the experiment; group IV: DOX group (n = 8); On Day 7 of the experiment, a single dose of 15 mg/kg intraperitoneally DOX injected; group V: DOX + THQ group (n = 8); Throughout the experiment, 10 mg/kg THQ per day and intraperitoneally 15 mg/kg DOX on Day 7 were injected. Immunohistochemically, tumor necrosis factor-α (TNF-α), interleukin-17 (IL-17), hypoxia-inducible factor 1α (HIF1-α), glucose regulatory protein 78 (GRP78), and the gene inducible by growth arrest and DNA damage 153 (GADD153) proteins were evaluated in the brain cortex, medulla, and hippocampus regions. Total oxidant status (TOS) levels and total antioxidant status (TAS) in the brain tissue were measured. TNF-α, IL-17, HIF1-α, GRP78, and GADD153 immunoreactivities significantly increased in the DOX group in the study. THQ significantly reduced these values. THQ increased the TAS level significantly and decreased the TOS level significantly compared to the DOX group. THQ may play a role as a neuroprotective agent in DOX-induced neurotoxicity in the cortex, medulla, and hippocampus regions of the brain.

中文翻译：

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百里醌对多柔比星引起的大脑皮层、髓质和海马中的炎症、氧化应激和内质网应激具有神经保护作用

尽管阿霉素 (DOX) 用于许多癌症治疗，但它会引起神经毒性。在这项研究中，评估了强效抗氧化剂百里醌 (THQ) 对 DOX 诱导的神经毒性的影响。总共使用了40只大鼠，并形成了5组。第一组：对照组（n  = 8）；第二组：橄榄油组（n  = 8）；第三组：THQ 组（n  = 8）；在整个实验过程中，每天腹膜内 (ip) 给予 THQ 10 mg/kg；第四组：DOX 组（n  = 8）；实验第7天，单剂量15mg/kg腹腔注射DOX；第五组：DOX + THQ 组（n = 8); 在整个实验过程中，每天注射 10 mg/kg THQ，并在第 7 天腹膜内注射 15 mg/kg DOX。免疫组织化学上，肿瘤坏死因子-α (TNF-α)、白细胞介素-17 (IL-17)、缺氧诱导因子 1α (HIF1-α)、葡萄糖调节蛋白 78 (GRP78) 以及生长停滞和 DNA 诱导的基因对大脑皮层、髓质和海马区的损伤 153 (GADD153) 蛋白进行了评估。测量脑组织中的总氧化状态 (TOS) 水平和总抗氧化状态 (TAS)。在研究中，DOX 组的 TNF-α、IL-17、HIF1-α、GRP78 和 GADD153 免疫反应性显着增加。THQ 显着降低了这些值。与 DOX 组相比，THQ 显着增加了 TAS 水平并显着降低了 TOS 水平。