Development and validation of an analytical method for the simultaneous determination of the alcohol biomarkers ethyl glucuronide, ethyl sulfate, N-acetyltaurine, and 16:0/18:1-phosphatidylethanol in human blood,Drug Testing and Analysis

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Development and validation of an analytical method for the simultaneous determination of the alcohol biomarkers ethyl glucuronide, ethyl sulfate, N-acetyltaurine, and 16:0/18:1-phosphatidylethanol in human blood
Drug Testing and Analysis ( IF 2.6 ) Pub Date : 2021-08-16 , DOI: 10.1002/dta.3147
Vanessa Hofmann ₁ , Tom R Sundermann ₁ , Georg Schmitt ₁ , Marc Bartel ₁

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As alcohol is the most common addictive substance worldwide, it is inevitable to advance the established research. New and more substantial analytical methods can be applied to reply to complex questions in legal or forensic contexts. Therefore, an analytical method for the simultaneous determination of four different alcohol biomarkers—ethyl glucuronide, ethyl sulfate, N-acetyltaurine, and 16:0/18:1-phosphatidylethanol—in human blood was developed, validated, and verified. Despite the different chemical properties of the analytes, a specific determination via HPLC–MS/MS was achieved using a novel type of a Phenomenex Luna® Omega Sugar column. Furthermore, all criteria for a successful validation were fulfilled according to forensic guidelines. The method proved to be linear and demonstrates selectivity and sufficient sensitivity for every biomarker. LODs obtained with this method of 2.6 ng/ml (EtG), 4.7 ng/ml (EtS), 12.5 ng/ml (NAcT), and 6.9 ng/ml (PEth) were in an acceptable range for routine applications, and the stability of all analytes over a range of 12 h is given. The verification of the new developed method was performed with authentic samples. Thus, whole blood and postmortem samples were analyzed to obtain information about the drinking behavior, which can answer complex questions regarding alcohol consumption.

中文翻译：

一种同时测定人体血液中酒精生物标志物乙基葡萄糖醛酸、硫酸乙酯、N-乙酰牛磺酸和 16:0/18:1-磷脂酰乙醇的分析方法的开发和验证

由于酒精是世界范围内最常见的成瘾物质，因此推进已建立的研究是不可避免的。可以应用新的和更实质性的分析方法来回答法律或法医环境中的复杂问题。因此，开发、验证和验证了一种同时测定人体血液中四种不同的酒精生物标志物——葡萄糖醛酸乙酯、硫酸乙酯、N-乙酰牛磺酸和 16:0/18:1-磷脂酰乙醇的分析方法。尽管分析物的化学性质不同，但使用新型 Phenomenex Luna® Omega Sugar 色谱柱可通过 HPLC-MS/MS 进行特定测定。此外，成功验证的所有标准都根据法医指南得到满足。该方法被证明是线性的，并证明了对每种生物标志物的选择性和足够的灵敏度。用这种方法获得的 LOD 分别为 2.6 ng/ml (EtG)、4.7 ng/ml (EtS)、12.5 ng/ml (NAcT) 和 6.9 ng/ml (PEth)，在常规应用的可接受范围内，稳定性给出了 12 小时范围内的所有分析物的值。使用真实样品对新开发的方法进行了验证。因此，对全血和死后样本进行分析以获得有关饮酒行为的信息，这可以回答有关饮酒的复杂问题。使用真实样品对新开发的方法进行了验证。因此，对全血和死后样本进行分析以获得有关饮酒行为的信息，这可以回答有关饮酒的复杂问题。使用真实样品对新开发的方法进行了验证。因此，对全血和死后样本进行分析以获得有关饮酒行为的信息，这可以回答有关饮酒的复杂问题。

更新日期：2021-08-16

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