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Multi-enzymatic recovery of fungal cellulases (Aspergillus niger) through solid-state fermentation of sugarcane bagasse
The Canadian Journal of Chemical Engineering ( IF 1.6 ) Pub Date : 2021-08-15 , DOI: 10.1002/cjce.24292
Tales Prado Alves 1 , Carina Contini Triques 1 , Edson Antonio da Silva 1 , Marcia R. Fagundes‐Klen 1 , Salah Din Mahmud Hasan 1
Affiliation  

In this study, the optimum conditions for the multi-enzymatic recovery of cellulases produced by Aspergillus niger were investigated using sugarcane bagasse. Two extraction methods were investigated: the two-stage solid–liquid extraction (SLE) followed by ultrasound-assisted extraction (UAE), and the single-stage SLE. The ultrasound effects were evaluated using a Doehlert design, in which the pH (5.0–9.0) and sonication power (0.8–2.0 W ml−1) were independent variables. For the single-stage SLE, temperature (25–45°C), time (10–60 min), and pH (5.0–9.0) were analyzed using the Box–Behnken design. Both processes were monitored to evaluate FPase, CMCase, and β-glucosidase (U ml−1) activities. The maximum enzymatic activities (EA) obtained for FPase, CMCase, and β-glucosidase in the SLE–UAE were 0.352, 0.321, and 1.412 U ml−1, respectively. Unlike in most previous studies, sonication was insignificant (p < 0.05) with respect to the enzymatic complex within the evaluated ranges. Moreover, sonication changed the EA when lower than 1.2 and higher than 1.6 W ml−1, mainly inhibiting the EA of β-glucosidase. The single-stage SLE was more effective than the two-stage SLE–UAE, and the maximum EA values for FPase, CMCase, and β-glucosidase were 0.354, 0.303, and 3.135 U ml−1, respectively. The single-stage process was better because it consumed less energy, required simpler equipment, and provided higher efficiency in a shorter time. This study will improve diversified enzyme extraction from sugarcane bagasse, reduce enzyme production costs, and enhance bagasse utilization.

中文翻译:

甘蔗渣固态发酵多酶法回收真菌纤维素酶(黑曲霉)

在这项研究中,使用甘蔗渣研究了多酶法回收黑曲霉产生的纤维素酶的最佳条件。研究了两种萃取方法:两阶段固液萃取 (SLE),然后是超声辅助萃取 (UAE),以及单阶段 SLE。使用 Doehlert 设计评估超声效果,其中 pH (5.0–9.0) 和超声功率 (0.8–2.0 W ml -1 ) 是独立变量。对于单级 SLE,使用 Box-Behnken 设计分析温度 (25–45°C)、时间 (10–60 分钟) 和 pH (5.0–9.0)。监测这两个过程以评估 FPase、CMCase 和 β-葡萄糖苷酶 (U ml -1) 活动。在 SLE-UAE 中获得的 FPase、CMCase 和 β-葡萄糖苷酶的最大酶活性 (EA) 分别为 0.352、0.321 和 1.412 U ml -1。与大多数先前的研究不同,超声处理 对于评估范围内的酶复合物而言是微不足道的 ( p < 0.05)。此外,超声处理在低于1.2和高于1.6 W ml -1时改变了EA ,主要抑制了β-葡萄糖苷酶的EA。单阶段 SLE 比两阶段 SLE-UAE 更有效,FPase、CMCase 和 β-葡萄糖苷酶的最大 EA 值为 0.354、0.303 和 3.135 U ml -1, 分别。单级工艺更好,因为它消耗的能量更少,需要更简单的设备,并且在更短的时间内提供更高的效率。本研究将改进从甘蔗渣中提取多种酶,降低酶生产成本,提高甘蔗渣利用率。
更新日期:2021-08-15
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