Amplification-free genome analysis can revolutionize biology and medicine by uncovering genetic variations among individuals. Here, the authors report on a 3D-integrated nanopore for electrolysis to in situ detection of single-molecule DNA in a cell by ionic current measurements. It consists of a SiO₂ multipore sheet and a SiN_x nanopore membrane stacked vertically on a Si wafer. Single cell lysis is demonstrated by 10⁶ V m⁻¹-level electrostatic field focused at the multinanopore. The intracellular molecules are then directly detected as they move through a sensing zone, wherein the authors find telegraphic current signatures reflecting folding degrees of freedom of the millimeter-long polynucleotides threaded through the SiN_x nanopore. The present device concept may enable on-chip single-molecule sequencing to multi-omics analyses at a single-cell level.

中文翻译：

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使用三维集成纳米孔检测细胞中的单分子脱氧核糖核酸

无扩增基因组分析可以通过揭示个体之间的遗传变异来彻底改变生物学和医学。在这里，作者报告了一种用于电解的 3D 集成纳米孔，可通过离子电流测量原位检测细胞中的单分子 DNA。它由SiO ₂多孔片和SiN _x纳米孔膜垂直堆叠在Si晶片上组成。单细胞裂解由聚焦在多纳米孔的 10 ⁶  V m ^{-1级静电场证明。}然后当细胞内分子通过传感区时直接检测它们，其中作者发现电报电流特征反映穿过 SiN 的毫米长多核苷酸的折叠自由度_x纳米孔。本装置概念可以使芯片上的单分子测序能够在单细胞水平上进行多组学分析。