Gastric cancer is a common malignant cancer, which is one of the most affected cancers by PI3K/AKT signaling. Here, we investigated the anti-tumor role of Astragalin, a natural flavonoid compound, in gastric cancer and explored the underlying molecular mechanism. Three well-established gastric cancer cell lines and xenograft mouse model were used to examine the anti-tumor effect of Astragalin by using CCK-8, transwell assays, and Western blot. Tumor burden of xenograft mice with Astragalin administration was monitored and determined during and at end of the experiments. Astragalin could effectively inhibit cell viability of gastric cancer cells and possessed good anti-tumor activity in xenograft mice. In addition, astragalin induced the expression of apoptotic signaling proteins, suppressed the migration and invasion cancer cells, and inhibited the PI3K/AKT signaling pathway significantly. In contrast, epidermal growth factor stimulation was able to block the anti-tumor activity of Astragalin. In conclusion, astragalin exerts its anticancer activities through inhibiting PI3K/AKT signaling, which highlights its potential for the treatment of gastric cancer.

中文翻译：

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黄酮类黄芪素在胃癌中显示出抗肿瘤活性并抑制 PI3K/AKT 信号传导

胃癌是一种常见的恶性肿瘤，是受PI3K/AKT信号影响最大的癌症之一。在这里，我们研究了黄芪甲苷（一种天然类黄酮化合物）在胃癌中的抗肿瘤作用，并探索了潜在的分子机制。使用三种成熟的胃癌细胞系和异种移植小鼠模型，通过 CCK-8、transwell 检测和蛋白质印迹检测黄芪甲苷的抗肿瘤作用。在实验期间和实验结束时监测和确定给予黄芪甲苷的异种移植小鼠的肿瘤负荷。黄芪甲苷能有效抑制胃癌细胞的细胞活力，在异种移植小鼠中具有良好的抗肿瘤活性。此外，黄芪甲苷可诱导凋亡信号蛋白的表达，抑制癌细胞的迁移和侵袭，并显着抑制PI3K/AKT信号通路。相反，表皮生长因子刺激能够阻断黄芪甲苷的抗肿瘤活性。总之，黄芪甲苷通过抑制PI3K/AKT信号发挥其抗癌活性，这凸显了其治疗胃癌的潜力。