Cervical cancer (CC) is one of the most common and malignant tumors in women. In this study, we aim to explore the role and mechanism of F-box and leucine rich repeat protein 19 antisense RNA 1 (FBXL19-AS1), a novel long-chain non coding RNA (lncRNA) with marked roles in a variety of tumors, in regulating the proliferation and metastasis of CC. The expression of FBXL19-AS1, miR-193a-5p and COL1A1 were detected by RT-PCR and western blot. Gain- and loss-of functional assays of FBXL19-AS1 and miR-193a-5p were performed in CC cell lines in vitro or in vivo. The proliferation, migration, invasion, apoptosis and epithelial-mesenchymal transition (EMT) of CC cells were determined. FBXL19-AS1 and COL1A1 were significantly up-regulated in CC tissues, while miR-193a-5p was significantly down-regulated. Overexpression of FBXL19-AS1 significantly promoted the proliferation, migration, invasion, EMT and growth of CC cells and inhibited apoptosis, while knockdown of FBXL19-AS1 had the opposite effects. On the other hand, miR-193a-5p inhibited the proliferation and metastasis of CC cells. Mechanistically, FBXL19-AS1 functioned as a competitive endogenous RNA (ceRNA) and inhibited the expression of miR-193a-5p, which targeted at the 3’-UTR site of COL1A1 and negatively regulated COL1A1 expression. FBXL19-AS1 promotes the proliferation and metastasis of CC cells by sponging miR-193a-5p and up-regulating COL1A1.

中文翻译：

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LncRNA FBXL19-AS1通过上调COL1A1作为miR-193a-5p的海绵促进宫颈癌的增殖和转移

宫颈癌（CC）是女性最常见的恶性肿瘤之一。在本研究中，我们旨在探索 F-box 和富含亮氨酸重复蛋白 19 反义 RNA 1 (FBXL19-AS1) 的作用和机制，这是一种在多种肿瘤中具有显着作用的新型长链非编码 RNA (lncRNA)。 , 在调节 CC 的增殖和转移中。RT-PCR和蛋白质印迹检测FBXL19-AS1、miR-193a-5p和COL1A1的表达。FBXL19-AS1 和 miR-193a-5p 的获得和丧失功能测定在体外或体内 CC 细胞系中进行。测定CC细胞的增殖、迁移、侵袭、凋亡和上皮间质转化(EMT)。FBXL19-AS1 和 COL1A1 在 CC 组织中显着上调，而 miR-193a-5p 显着下调。FBXL19-AS1的过表达显着促进CC细胞的增殖、迁移、侵袭、EMT和生长并抑制细胞凋亡，而FBXL19-AS1的敲低则具有相反的作用。另一方面，miR-193a-5p抑制CC细胞的增殖和转移。从机制上讲，FBXL19-AS1 作为竞争性内源 RNA (ceRNA) 起作用并抑制 miR-193a-5p 的表达，miR-193a-5p 靶向 COL1A1 的 3'-UTR 位点并负调节 COL1A1 表达。FBXL19-AS1通过海绵化miR-193a-5p和上调COL1A1促进CC细胞的增殖和转移。从机制上讲，FBXL19-AS1 作为竞争性内源 RNA (ceRNA) 起作用并抑制 miR-193a-5p 的表达，miR-193a-5p 靶向 COL1A1 的 3'-UTR 位点并负调节 COL1A1 表达。FBXL19-AS1通过海绵化miR-193a-5p和上调COL1A1促进CC细胞的增殖和转移。从机制上讲，FBXL19-AS1 作为竞争性内源 RNA (ceRNA) 起作用并抑制 miR-193a-5p 的表达，miR-193a-5p 靶向 COL1A1 的 3'-UTR 位点并负调节 COL1A1 表达。FBXL19-AS1通过海绵化miR-193a-5p和上调COL1A1促进CC细胞的增殖和转移。