We have investigated the mode of action of synthetic biocides, (2-(thiocyanomethylthio) benzothiazole(TCMTB), dichlorophen, (commonly used in leather industry for preservation) and natural biocides, oregano and eucalyptus oils, on Aeromonas hydrophila using Raman spectroscopy in collaboration with multivariate analysis and 2D correlation spectroscopy to evaluate whether Raman spectra acquired contained valuable information to study the action of biocides on bacterial cells. The growth of A. hydrophila in clear and outer edge zone of inhibition differ in their reaction with different biocides, which allows us to highlight the differences as a characteristic of two kinds of bacteria. Such classification helps identify oregano oil as the most effective biocide by altering clear and outer edge zone of bacteria. Standard disk diffusion assay method was used for screening biocide bacteria interactions and later analysed by Raman spectroscopy. The paper also presents the introduction of TCMTB and oregano oil into leather processing stages to examine and determine the antimicrobial effect as an application to real-world setting. Therefore, we conclude that Raman spectroscopy with appropriate computational tools constitutes a powerful approach for screening biocides, which provide solutions to all the industries using biocides including leather industry, considering the potentially harmful effect of biocides to humans and the environment.

中文翻译：

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通过拉曼光谱和化学计量学监测合成和天然杀菌剂对嗜水气单胞菌的作用模式

标准圆盘扩散测定方法用于筛选杀生物剂细菌相互作用，随后通过拉曼光谱进行分析。该论文还介绍了将 TCMTB 和牛至油引入皮革加工阶段，以检查和确定抗菌效果，并将其应用到实际环境中。因此，我们得出结论，考虑到杀菌剂对人类和环境的潜在危害，拉曼光谱与适当的计算工具构成了筛选杀菌剂的强大方法，为包括皮革工业在内的所有使用杀菌剂的行业提供了解决方案。该论文还介绍了将 TCMTB 和牛至油引入皮革加工阶段，以检查和确定抗菌效果，并将其应用到实际环境中。因此，我们得出结论，考虑到杀菌剂对人类和环境的潜在危害，拉曼光谱与适当的计算工具构成了筛选杀菌剂的强大方法，为包括皮革工业在内的所有使用杀菌剂的行业提供了解决方案。该论文还介绍了将 TCMTB 和牛至油引入皮革加工阶段，以检查和确定抗菌效果，并将其应用到实际环境中。因此，我们得出结论，考虑到杀菌剂对人类和环境的潜在危害，拉曼光谱与适当的计算工具构成了筛选杀菌剂的强大方法，为包括皮革工业在内的所有使用杀菌剂的行业提供了解决方案。