M. morganii is a gram-negative, non-lactose fermenting and an opportunistic pathogen frequently associated with nosocomial infections. Although first isolated in 1906 from a pediatric fecal sample, not many M. morganii isolates have been sequenced. The objective of this work is to determine the complete genome sequence of an XDR M. morganii strain (SMM01) isolated from the urine of a patient with urinary and fecal incontinence and to characterize its antimicrobial resistance profile. Here, we report the complete genome sequence of M. morganii SMM01 generated from the hybrid assembly of Illumina HiSeq X and Nanopore MinION reads. The assembly is 100% complete with genome size of 39,30,130 bp and GC content of 51%. Genomic features include 3617 CDS, 18 rRNAs, 78 tRNAs, 4 ncRNAs and 60 pseudogenes. Antimicrobial resistance profile was characterized by the presence of genes conferring resistance to aminoglycosides, β-lactams, fluoroquinolones, chloramphenicol, and tetracyclines. Secondary metabolite biosynthetic gene clusters like NRPS, T1PKS, thiopeptide, beta-lactone, and bacteriocin were identified. The genome data described here would be the first complete genome of an Indian M. morganii isolate providing crucial information on antimicrobial resistance patterns, paving the way for further comparative genome analyses.

中文翻译：

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从尿失禁患者中分离出的广泛耐药 (XDR) M. morganii SMM01 的完整基因组序列

M. morganii 是一种革兰氏阴性非乳糖发酵菌，是一种经常与医院感染相关的机会性病原体。尽管在 1906 年首次从儿科粪便样本中分离出来，但对许多摩根菌分离株进行了测序。这项工作的目的是确定从尿失禁和大便失禁患者的尿液中分离出的 XDR M. morganii 菌株 (SMM01) 的完整基因组序列，并表征其抗菌素耐药性特征。在这里，我们报告了由 Illumina HiSeq X 和 Nanopore MinION 读数的混合组装生成的摩根氏菌 SMM01 的完整基因组序列。组装完成 100%，基因组大小为 39,30,130 bp，GC 含量为 51%。基因组特征包括 3617 个 CDS、18 个 rRNA、78 个 tRNA、4 个 ncRNA 和 60 个假基因。抗菌素耐药性的特点是存在对氨基糖苷类、β-内酰胺类、氟喹诺酮类、氯霉素和四环素类耐药的基因。鉴定了次级代谢物生物合成基因簇，如 NRPS、T1PKS、硫肽、β-内酯和细菌素。此处描述的基因组数据将是印度摩根菌分离株的第一个完整基因组，提供有关抗菌素耐药性模式的关键信息，为进一步的比较基因组分析铺平了道路。